|
| Status |
Public on Dec 19, 2013 |
| Title |
HS999 cells at passage 10 |
| Sample type |
genomic |
| |
|
| Source name |
human ES cells line HS999
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HS999
cell type: human ES cells
passage: passage 10
|
| Growth protocol |
Human Es cells were grown on laminin-521
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted using Qiagen DNA Mini Kit
|
| Label |
Biotin
|
| Label protocol |
According to Affymetrics instructions
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000/G
|
| Description |
HS999 p10
|
| Data processing |
The array image was acquired using Affymetrix GeneChip®Command Console 3.1. The CEL files were analyzed using Affymetrix Genotyping Console version 4.1.4.840. Copy Number/LOH analysis was performed according to the Genotyping Console manual, using a set of 75 healthy individuals as a reference (75_samples_ref.REF).
CNCHP files containing information on the observed copy number of genomic segments. One REF file produced by the Batch Sample Mode of copy number analysis in the Affymetrix Genotyping Console.
|
| |
|
| Submission date |
Dec 18, 2013 |
| Last update date |
Dec 19, 2013 |
| Contact name |
Sergey Rodin |
| Organization name |
Karolinska institute
|
| Street address |
Scheelesvag 2, Plan 4:B1
|
| City |
Stockholm |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE53436 |
Affymetrix SNP array data for various human ES cell lines |
|
