|
| Status |
Public on Mar 14, 2014 |
| Title |
Adoptive transfer of OT-I derived T cells transduced with shRNA into B16 tumor bearing mice_2-sp2 |
| Sample type |
RNA |
| |
|
| Source name |
CD8 T cells isolated from spleen
|
| Organism |
Mus musculus |
| Characteristics |
cell type: CD8 T cells
transduction: shRNA targeting Ppp2r2d
tissue: spleen
sample name: 2-sp2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol, per manufacturers protocol
|
| Label |
Biotin
|
| Label protocol |
One cycle amplification by standard Affymetrix procedure
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
CD8 T cells isolated from spleen; transduced with shRNA targeting: Ppp2r2d; Sample name: 2-sp2
Adoptive transfer of OT-I derived T cells transduced with shRNA into B16 tumor bearing mice.
|
| Data processing |
JMP Genomics
Background correction: RMA; Transformation: log2; Normalization: median scaling; Summary method: median
|
| |
|
| Submission date |
Dec 17, 2013 |
| Last update date |
Mar 14, 2014 |
| Contact name |
John R Walker |
| E-mail(s) |
jwalker@gnf.org
|
| Phone |
858-812-1636
|
| Organization name |
Genomics Institute of the Novartis Research Foundation
|
| Lab |
Genetics Core
|
| Street address |
10675 John Jay Hopkins
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE53388 |
Immunotherapy Targets in Tumors |
|
