|
| Status |
Public on Mar 31, 2014 |
| Title |
GS-9_IFNb300_6h_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
GS-9_IFNb300_6h_rep1
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: glioma
cell line: GS-9
|
| Treatment protocol |
IFN-beta was dissolved in ddH2O. Cells were exposed to either IFN 300 IU/ml or ddH2O for either 6 h or 24 h before assembly of the cell pellets.
|
| Growth protocol |
LNT-229 cells were grown in DMEM with 10% FCS and glutamine; GS-cells were grown in Neurobasal medium supplemented with growth factors and B27.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the miRNeasy Mini Kit (Qiagen, Hilden, Germany, Cat. No. 217004); RNA qualitiy was checked with the 2100 BioAnalyzer (Agilent, Santa Clara, USA)
|
| Label |
Biotin
|
| Label protocol |
Sample preparation was done with 100 ng total-RNA using the GeneChip® 3´ IVT Express Kit (PN 901229) from Affymetrix (Santa Clara, USA) according to the instructions of the Affymetrix GeneChip® 3´ IVT Express Technical Manual (PN 702646). 10 µg of the fragmented cRNA were hybridized on the Gene Chip® Human Genome U133A 2.0 Arrays (Affymetrix, PN 900469)
|
| |
|
| Hybridization protocol |
Samples were hybridized for 16h at 45°C using Hybridization Kit materials (PN 900720, Affymetrix) according to the GeneChip® 3’ IVT Express Kit Protocol (PN 702646 Rev. 6, Affymetrix)
|
| Scan protocol |
Affymetrix Gene Chip Scanner 3000 7G; AGCC
|
| Data processing |
Quantile normalization / RMA summarization (GeneSpring 12.1, Agilent)
|
| |
|
| Submission date |
Dec 11, 2013 |
| Last update date |
Mar 31, 2014 |
| Contact name |
Karl Koehrer |
| E-mail(s) |
rene.deenen@hhu.de
|
| Organization name |
University of Duesseldorf
|
| Department |
BMFZ
|
| Lab |
GTL
|
| Street address |
Universitaetsstr. 1
|
| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE53213 |
Expression data from glioma cells exposed to interferon (IFN)-beta |
|
