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Sample GSM1278113 Query DataSets for GSM1278113
Status Public on Oct 15, 2014
Title serum_healthy control_4
Sample type RNA
 
Source name serum
Organism Homo sapiens
Characteristics samples of different individuals pooled?: no
age: 50
gender: male
als mutation: /
Treatment protocol Serum samples were processed within 1 h after blood drawing and stored at -80° C until further usage.
Extracted molecule total RNA
Extraction protocol Total RNA from 8-10 ml of serum were extracted using the QIAzol lysis reagent (Qiagen) according to the manufacturer's instructions. The aqueous phase was collected and total RNA precipitated by addition of 1.4 volumes of isopropanol and overnight incubation at -20°C. Precipitated RNA was redissolved in water and purified a second time using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol FlashTagTM Biotin HSR RNA Labeling Kit (Genisphere, LLC, Hatfield, PA, USA) according to the instructions of the manufacturer
 
Hybridization protocol according to the instructions in the FlashTagTM Biotin HSR RNA Labeling Kit
Scan protocol according to Affymetrix protocols using a GeneChip 3000 Scanner
Description control for comparison with sALS, fALS and carrier
Data processing Affymetrix Expression Console 1.3.1. for normalization (RMA+DBAG; human only); BRB Array tools for statistical analysis
 
Submission date Dec 03, 2013
Last update date Oct 15, 2014
Contact name Karlheinz Holzmann
E-mail(s) karlheinz.holzmann@uni-ulm.de
Organization name Ulm University
Department Medical Faculty
Lab Genomics Core Facility
Street address Helmholtzstreet 8/1
City Ulm
ZIP/Postal code D-89081
Country Germany
 
Platform ID GPL16384
Series (1)
GSE52917 Early pre-symptomatic and progressive signatures of non-coding RNAs in ALS patients and mutation carriers

Data table header descriptions
ID_REF
VALUE RMA normalized, log2

Data table
ID_REF VALUE
14q0_st 0.7650844
14qI-1_st 1.161961
14qI-1_x_st 1.321196
14qI-2_st 0.7370405
14qI-3_x_st 0.668921
14qI-4_st 0.7622386
14qI-4_x_st 0.9085484
14qI-5_st 0.7500465
14qI-6_st 0.7224156
14qI-6_x_st 0.8526413
14qI-7_st 0.8121908
14qI-8_st 1.030048
14qI-8_x_st 0.536693
14qI-9_x_st 0.8123817
14qII-10_st 0.5343997
14qII-10_x_st 0.8306155
14qII-11_st 0.9025581
14qII-11_x_st 0.7407796
14qII-12_st 0.8423908
14qII-12_x_st 0.914253

Total number of rows: 5683

Table truncated, full table size 153 Kbytes.




Supplementary file Size Download File type/resource
GSM1278113_Co4.CEL.gz 676.8 Kb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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