|
| Status |
Public on Jun 04, 2015 |
| Title |
R444ST |
| Sample type |
RNA |
| |
|
| Source name |
Cervical cancer biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
clinic: Case
age (years): 71
histology: SCC
tumor stage: IIB
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated ssDNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, ssDNA were hybridized for 16 hr at 45C and 60 rpm on Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 protocol FS450_0007
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix) with GeneChip Scanner 3000
|
| Description |
Gene expression data from Cervical cancer biopsy
|
| Data processing |
Microarrays were normalized using the RMA algorithm (Robust Multichip Average) in the Affymetrix Expression Console. The analysis was done at Gene Level Default: RMA-Sketch
|
| |
|
| Submission date |
Dec 02, 2013 |
| Last update date |
Jun 04, 2015 |
| Contact name |
INGRID MEDINA MARTINEZ |
| E-mail(s) |
fiemed@hotmail.com
|
| Organization name |
HOSPITAL GENERAL DE MEXICO OD
|
| Department |
MEDICINA GENOMICA
|
| Lab |
MEDICINA GENOMICA
|
| Street address |
DR. BALMIS 148
|
| City |
MEXICO CITY |
| State/province |
CUAUHTEMOC |
| ZIP/Postal code |
06726 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE52903 |
Gene Dosage, Mainly 3q Amplification, Deregulates a Quarter of Genes in Cervical Cancer: It Induces Glycolysis, Anaphase-dependent Proteasome Proteolysis, and Low Survival |
| GSE52904 |
Impact of Gene Dosage on Gene Expression, Biological Processes and Survival in Cervical Cancer: a Genome-Wide Follow-Up Study |
|
