|
| Status |
Public on Mar 01, 2015 |
| Title |
tail-1875 |
| Sample type |
RNA |
| |
|
| Source name |
tail skin
|
| Organism |
Mus musculus |
| Characteristics |
tissue: tail skin
age: 8 weeks
hras_genotype: KO
plate: 2
mouse.no: 1875
microarray.outlier: no
Sex: M
|
| Treatment protocol |
Mice were aged to 8 weeks, untreated
|
| Growth protocol |
Mice were housed in standard conditions with standard chow and water ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol followed by Qiagen RNEasy clean-up
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
|
| |
|
| Hybridization protocol |
90 microliters of hybridization sample were incubated in the Affymetrix GeneTitan Hybridization Oven instrument for 16 hours at 48C
|
| Scan protocol |
Arrays were processed with the Affymetrix GeneTitan Fluidics Station instrument, using the MoGene-1_1-st-v1-96 fluidics script and GeneTitan Hybridization, Wash, and Stain Kit for WT Array Plates. Arrays were scanned in the Affymetrix GeneTitan Multi-Channel (MC) instrument with the AGCC GeneTitan Instrument Control.
|
| Description |
Gene expression data from untreated P56 mouse skin
|
| Data processing |
CEL files were background corrected and normalized with RMA using oligo in R and a custom probe database which avoids SNPs in FVB and Spretus genomes. Data were batch-corrected for plate and genotype and then log2 transformed.
|
| |
|
| Submission date |
Nov 21, 2013 |
| Last update date |
Mar 01, 2015 |
| Contact name |
David Quigley |
| Organization name |
UCSF
|
| Department |
Helen Diller Comprehensive Cancer Center
|
| Lab |
Ashworth Lab
|
| Street address |
1450 Third St. Room 207
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
|
