|
| Status |
Public on Mar 28, 2014 |
| Title |
naïve bone marrow-GMP_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
strain: C57Bl/6
genotype/variation: wild-type
tissue: bone marrow
tissue treatment: control-cDNA
cell type: GMP cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Microarrays were washed at low (6X SSPE) and high (100mM MES, 0.1M NaCl) stringency and stained with streptavidin-phycoerythrin. Fluorescence was amplified by adding biotinylated anti-streptavidin and an additional aliquot of streptavidin-phycoerythrin stain
|
| |
|
| Hybridization protocol |
cDNA was amplied with NuGen WT Ovation Pico kit and hybridized to Affymetrix GeneChip Mouse Gene 1.0 ST microarrays at the University of Pennsylvania’s Microarray facility.
|
| Scan protocol |
A confocal scanner was used to collect fluorescence signal at 3um resolution after excitation at 570 nm. The average signal from two sequential scans was calculated for each microarray feature.
|
| Description |
BM-GMP2
|
| Data processing |
Affymetrix Power Tools were used to process and quantile normalize fluorescent hybridization signals using Robust Multichip Averaging. Transcript values were log2 normalized.
|
| |
|
| Submission date |
Nov 18, 2013 |
| Last update date |
Mar 28, 2014 |
| Contact name |
Mark C. Siracusa |
| E-mail(s) |
msira@mail.med.upenn.edu
|
| Organization name |
UPenn
|
| Department |
Microbiology
|
| Lab |
Artis
|
| Street address |
421 Curie Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE52485 |
Gene expression profiling of naïve bone marrow-resident granulocyte monocyte precursors (GMPs) and TSLP-elicited splenic GMP-like cells |
|
