|
| Status |
Public on Jan 21, 2014 |
| Title |
Epi_Cast_Vehicle12_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
adult male
|
| Organism |
Mus musculus |
| Characteristics |
tissue: caput epididymis
|
| Treatment protocol |
Tissues were harvested from adult castrated male mice 12 or 24 hours after single testosterone- or vehicle-injection.
|
| Growth protocol |
Mice were housed in standard 12-h light-dark cycle and were fed ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit with DNaseI treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2, 12-h vehicle-treatment
9323675032_F
|
| Data processing |
The data were normalised using quantile normalisation with BeadStudio Program from Illumina.
|
| |
|
| Submission date |
Nov 05, 2013 |
| Last update date |
Jan 21, 2014 |
| Contact name |
Olli A. Jänne |
| E-mail(s) |
olli.janne@helsinki.fi
|
| Phone |
+358919125040
|
| Organization name |
University of Helsinki
|
| Department |
Inst. of Biomedicine/Physiology
|
| Lab |
Androgen Receptor Laboratory
|
| Street address |
Haartmaninkatu-8
|
| City |
Helsinki |
| ZIP/Postal code |
FI-00014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE47181 |
Tissue-specific pioneer factors associate with androgen receptor cistromes and transcription programs. [microarray] |
| GSE47194 |
Tissue-specific pioneer factors associate with androgen receptor cistromes and transcription programs |
|
