|
| Status |
Public on Nov 30, 2013 |
| Title |
J1 NT siRNA_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse J1 ESC
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic cell line
cell line: J1
transfection: non-targeting siRNA
|
| Treatment protocol |
Cell were transfected with siRNAs against NT, Thoc2 and Thoc5. Total RNA was harvested 96 hours after transfection.
|
| Growth protocol |
J1 cell were maintianed in ESGRO medium. Cell were cultured in M10 medium when transfection or infection was performed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were prepared using Therno RNA kit according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Labeling was carried out with the Agilent Low Input Quick Amp Labeling Kit, one color, using 1 ug total RNA.
|
| |
|
| Hybridization protocol |
Hybridization was carried out on Agilent Mouse GE 4x44K v2 Microarray (G2519F) accrording to Agilent protocol.
|
| Scan protocol |
Arrays were scanned on Agilent SureScan Microarray Scanner according to manufacturer's instructions using the one color scan setting.
|
| Data processing |
The scanned images were analyzed with Agilent Feature Extraction Software 11.0 using default parameters to obtain background subtracted Processed Signal intensities.
|
| |
|
| Submission date |
Oct 21, 2013 |
| Last update date |
Nov 30, 2013 |
| Contact name |
Li Wang |
| Organization name |
NIH
|
| Street address |
111 T.W. Alexander Dr
|
| City |
Durham |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL4134 |
| Series (2) |
| GSE51496 |
The THO complex regulates pluripotency gene mRNA export to control embryonic stem cell self-renewal and somatic cell reprogramming (microarray) |
| GSE51534 |
The THO complex regulates pluripotency gene mRNA export to control embryonic stem cell self-renewal and somatic cell reprogramming |
|
