|
| Status |
Public on Jul 27, 2007 |
| Title |
brain_Down_syndrome_sampleid_jg1737 |
| Sample type |
RNA |
| |
|
| Source name |
human post-mortem brain tissue, Down syndrome
|
| Organism |
Homo sapiens |
| Characteristics |
Disease_status: Down syndrome
Age (years): 76
Gender: Female
Post mortem interval (hours): 18
Braak stage: VI
Cause of death: Unknown
|
| Biomaterial provider |
Prof. Anthony Holland, Cambridge Brain Bank, Department of Pathology, Addenbrooke's Hospital, University of Cambridge, UK
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from human postmortem brain tissue using Trizol (Invitrogen, USA) or Tri reagent (Sigma, UK) total RNA isolation kits according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Double-stranded cDNA was synthesised from 5 micrograms of total RNA using Superscript II double-stranded cDNA synthesis kit (Invitrogen, USA), purified, then in-vitro transcribed and labelled using BioArray HighYield RNA transcript Labeling Kit (Enzo Life Sciences, USA)
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 micrograms of cRNA were hybridised to Affymetrix Human Genome 133A GeneChips for 16 hours at 45oC with constant rotation at 60rpm. Chips were washed and stained according to manufacturer's instructions using the Affymetrix GeneChip Fluidics Station 400.
|
| Scan protocol |
Affymetrix HG-U133A GeneChips were scanned with the Agilent GeneArray Scanner
|
| Description |
none
|
| Data processing |
The raw data (CEL files) were read into R statistical package and normalised using the robust multi-chip average (RMA) method (Irizarray et al, 'Exploration, normalization and summaries of high density oligonucleotide array probe level data', Biostatistics, 2003).
|
| |
|
| Submission date |
Jul 25, 2006 |
| Last update date |
Apr 27, 2020 |
| Contact name |
Sabine Bahn |
| E-mail(s) |
sb209@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Lab |
Cambridge Centre for Neuropsychiatric Research
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 1QT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE5390 |
Expression profiling of human adult postmortem brain tissue from Down syndrome and healthy control subjects |
|
| Relations |
| Reanalyzed by |
GSE149466 |
