|
| Status |
Public on Sep 26, 2013 |
| Title |
129_liver_control_biological_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Liver, Control diet
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129S1/SvImJ
gender: male
tissue: liver
|
| Treatment protocol |
Starting at 8 weeks of age, mice were maintained on the control diet or were fed a 25% calorie restricted diet (Bio-Serv; Frenchtown, NJ, USA; Catalog# F05314)
|
| Growth protocol |
Male mice were purchased from Jackson Laboratory at six weeks of age and were individually housed and fed 84 kcal/week of a diet based on the AIN93M formulation for two weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Affymetrix Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
|
| Description |
Gene expression data from mice consuming control diet
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Sep 11, 2013 |
| Last update date |
Sep 26, 2013 |
| Contact name |
Jamie Barger |
| E-mail(s) |
jamie.l.barger@gmail.com
|
| Organization name |
LifeGen Technologies, LLC
|
| Street address |
510 Charmany Drive, Suite 263
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53719 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE50789 |
Gene expression in mouse liver in response to short-term calorie restriction |
|
