|
| Status |
Public on Jan 01, 2007 |
| Title |
sll1124_WT_1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
sll1124 mutant
|
| Organism |
Synechocystis sp. PCC 6803 |
| Characteristics |
sll1473 mutant
|
| Growth protocol |
0.5% CO2 at 30oC in BG-11
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy midi kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
RNA Fluorescence labeling core kit, M-MLV version Ver.2.0 (Takara Bio Inc.)
|
| |
|
| Channel 2 |
| Source name |
Wild type strain
|
| Organism |
Synechocystis sp. PCC 6803 |
| Characteristics |
Wild type strain
|
| Growth protocol |
0.5% CO2 at 30oC in BG-11
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy midi kit (Qiagen)
|
| Label |
Cy5
|
| Label protocol |
RNA Fluorescence labeling core kit, M-MLV version Ver.2.0 (Takara Bio Inc.)
|
| |
|
| |
| Hybridization protocol |
65oC, overnight
|
| Scan protocol |
ScanArray 4000 (PerkinElmer, Inc., MA, USA)
|
| Description |
16S and 23S rRNA data was excluded from the analysis.
|
| Data processing |
Signal of each individual spot was subtracted by background (average signal of corners of each spot) and divided by average value of all spot.
|
| |
|
| Submission date |
Jul 24, 2006 |
| Last update date |
Jul 31, 2006 |
| Contact name |
Mitsunori Katayama |
| E-mail(s) |
katayama@bio.c.u-tokyo.ac.jp
|
| Organization name |
University of Tokyo
|
| Street address |
3-8-1 Komaba
|
| City |
Meguro |
| State/province |
Tokyo |
| ZIP/Postal code |
153-8902 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4017 |
| Series (1) |
| GSE5391 |
Examination of gene expression levels in mutants of phytochrome-related protain |
|
