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Sample GSM1220560 Query DataSets for GSM1220560
Status Public on Sep 18, 2013
Title Histone H3K27ac ChIP-Seq of CD4 Primary Cells; Histone.DS21777
Sample type SRA
 
Source name flow sorted CD4+ Cells,year 21; Histone.DS21777
Organism Homo sapiens
Characteristics sra sample accession: SRS165630
disease: None
biomaterial_provider: FHCRC HEIMFELD
biomaterial_type: Primary Cell
cell_type: CD4 Primary Cells
markers: CD4+
donor_id: RO 01679
donor_age: year 21
donor_health_status: NA
donor_sex: Male
donor_ethnicity: Caucasian
passage_if_expanded: NA
experiment_type: Histone H3K27ac
extraction_protocol: http://www.roadmapepigenomics.org/protocols/type/experimental
extraction_protocol_type_of_sonicator: Diagenode Bioruptor
extraction_protocol_sonication_cycles: 20-40 cycles of 0.5 minute on and 0.5 minute off
chip_protocol: http://www.roadmapepigenomics.org/protocolstype/experimental
chip_protocol_chromatin_amount: 60ug
chip_protocol_bead_type: Invitrogen Dynabeads
chip_protocol_bead_amount: 48ul
chip_protocol_antibody_amount: 3ug
chip_antibody: Histone H3K27ac
chip_antibody_provider: Active Motif
chip_antibody_catalog: 39133
chip_antibody_lot: 21311004
Extracted molecule genomic DNA
Extraction protocol Library construction protocol: Single read - Illumina
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
 
Description sample_term_id: CL_0000492
assay_term_id: OBI_0000716
nucleic_acid_term_id: SO_0000352
Design description: Histone H3K27ac ChIP-Seq
Library name: Histone.DS21777
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FEXPERIMENT%2FEDACC.18621
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FSAMPLE%2FEDACC.5345
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For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
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Data processing Various levels of processed data files will be made available as this project proceeds.
 
Submission date Aug 30, 2013
Last update date May 15, 2019
Contact name Northwest REMC
E-mail(s) rharris1@bcm.tmc.edu
Organization name University of Washington
Street address -
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platform ID GPL11154
Series (1)
GSE18927 University of Washington Human Reference Epigenome Mapping Project
Relations
SRA SRX342308
BioSample SAMN00204016

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
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