|
| Status |
Public on Oct 02, 2014 |
| Title |
Hippocampus_fear_conditioned_24hours_rep9 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampus_fear_conditioned_24hours
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
age: 2 months old
Sex: male
|
| Treatment protocol |
To acclimate the mice to the experimenter each animal was handled daily for 3 days prior to contextual fear conditioning (FC). Handling consisted of manipulation of the animals for 1-2 minutes per mouse in the same room as the experimental setting without exposure to the context. The conditioning protocol entailed a single 2-second, 1.5-mA foot shock, terminating at 2.5 minutes after placement of the mouse in the chamber, starting at 10 am (ZT3) daily.
|
| Growth protocol |
Mouse Hippomcapus dissected from C57BL/6J mice. .C57BL/6J adult male mice (2 months of age) were obtained from Jackson laboratories and housed individually for a week on a 12 hr/12 hr light/dark schedule with lights on at 7 am (Zeitgeber time (ZT) 0). Food and water were available ad libitum throughout the experiment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hippocampal dissections were performed immediately following the behavioral treatment, and alternated between FC and control animals. Tissue was collected at 30 minutes (FC30), 4 hours (FC4), 12 hours (FC12) and 24 hours after FC (FC24) as well as 30 minutes after testing for retrieval of the memory (RT30). Testing was performed at 24 hours after training over a 5-minute interval. Tissue was immersed in RNAlater (Qiagen) and immediately frozen. Animals that were handled but not trained were dissected at the same time of day to control for variations due to circadian rhythms (CC30, CC4 andCC12). The protocol was repeated over the course of 2 weeks to obtain 9 animals (2 hippocampi) per group, so that 9 independent FC experiments were represented in each time point and all animals for each group were dissected at the exactly the same time of day.RNA extraction was performed using the RNAEasy Microarray Tissue kit from Qiagen
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 3 ug total RNA at the UPENN molecular profiling core using the Ambion WT Expression Kit
|
| |
|
| Hybridization protocol |
cRNA was hybridized to a Mouse Gene 1.1 ST 96-Array Plate using GeneTitan Hybridization, Wash and Stain Kit for WT Array Plates. The array plate was washed and stained in the GeneTitan multi-channel instrument.
|
| Scan protocol |
Gene 1.1 ST Array Plates were scanned using the GeneTitan® Multi-channel Instrument
|
| Data processing |
RMA normalization using Affymetrix powertools
|
| |
|
| Submission date |
Aug 28, 2013 |
| Last update date |
Oct 02, 2014 |
| Contact name |
Lucia Peixoto |
| E-mail(s) |
lucia.peixoto@wsu.edu
|
| Phone |
5093686764
|
| Organization name |
Washington State University, Spokane
|
| Department |
College of Medicine
|
| Street address |
PO Box 1495
|
| City |
Spokane |
| State/province |
WA |
| ZIP/Postal code |
99224 |
| Country |
USA |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE50423 |
Contextual fear conditioning in the mouse hippocampus |
|
