|
| Status |
Public on Jul 01, 2014 |
| Title |
Whole peripheral blood_PaxGene Blood RNATube, Sample 262 |
| Sample type |
RNA |
| |
|
| Source name |
Whole peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: MAL
ethnicity: CAU
plate: E
rna integrity score: 7.8
phase of study: Phase2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from Whole peripheral blood using Paxgene Blood RNA kit. Quality control was performed using Agilent's Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Illumina's BeadArray protocol
|
| |
|
| Hybridization protocol |
Illumina's BeadArray protocol
|
| Scan protocol |
Illumina's BeadArray protocol and GenomeStudio (Gene expression Module)
|
| Description |
CVD_B087
|
| Data processing |
The raw log2 transformed 14,111 probes that are expressed in both phase of CDGY studies were normalized utilizing Supervised Normalization of Microarray algorithm (SNM package is downloadable from bioconductor) fitting technical effects of plate and RNA quality, adjusting BMI, gender, ethnicity, and Age. CVD_TYPE was considered as a biological variable.
|
| |
|
| Submission date |
Aug 15, 2013 |
| Last update date |
Jul 01, 2014 |
| Contact name |
Jinhee Kim |
| E-mail(s) |
jinhee.kim@northwestern.edu
|
| Organization name |
Northwestern University
|
| Department |
Infectious Disease
|
| Lab |
Martinelli
|
| Street address |
676 North St. Clair Street Arkes Suite 2330
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE49925 |
Gene expression profiles associated with acute myocardial infarction and risk of cardiovascular death |
|
