|
| Status |
Public on Apr 21, 2014 |
| Title |
BRAF-V600E melanoma_451Lu_subline_drug resistant |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
451Lu_drug resistant
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BRAF-V600E melanoma 451Lu cell line
genotype/variation: isogenic drug resistant-derived subline
tissue origin: lung metastasis
age: 24 hrs
|
| Treatment protocol |
Drug resistant sublines were generated by chronic exposure to escaliting doses of trametinib
|
| Growth protocol |
Melanoma cells are grown in DMEM + 5% FBS as previously described (Villanueva, et al. Cancer Cell. 2010 Dec 14;18(6):683-95 - PMID: 21156289)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were scrapped, pelleted, and genomic DNA extracted using Qiagen genomic DNA purification kit.
|
| Label |
Cy5
|
| Label protocol |
Experimental and Control DNA was labeled using Invitrogen's BioPrime Total for Agilent aCGH following standard labeling and purification protocol.
|
| |
|
| Channel 2 |
| Source name |
reference sample
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Female Control DNA from Promega
|
| Treatment protocol |
Drug resistant sublines were generated by chronic exposure to escaliting doses of trametinib
|
| Growth protocol |
Melanoma cells are grown in DMEM + 5% FBS as previously described (Villanueva, et al. Cancer Cell. 2010 Dec 14;18(6):683-95 - PMID: 21156289)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were scrapped, pelleted, and genomic DNA extracted using Qiagen genomic DNA purification kit.
|
| Label |
Cy3
|
| Label protocol |
Experimental and Control DNA was labeled using Invitrogen's BioPrime Total for Agilent aCGH following standard labeling and purification protocol.
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed following Agilent's Array-based CGH for Genomic DNA Analysis - Enzymatic Labeling protocol, using reagents supplied in the Agilent Oligo aCGH Hybridization Kit.
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
|
| Description |
451Lu-MR
|
| Data processing |
Agilent Feature Extraction Software (version 10.5.1.1) was used for background subtraction and linear normalization.
|
| |
|
| Submission date |
Aug 01, 2013 |
| Last update date |
Apr 21, 2014 |
| Contact name |
Katherine Nathanson |
| E-mail(s) |
knathans@exchange.upenn.edu
|
| Organization name |
Perelman School of Medicine at the University of Pennsylvania
|
| Street address |
421 Curie Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19103 |
| Country |
USA |
| |
|
| Platform ID |
GPL9777 |
| Series (1) |
| GSE49430 |
Human BRAF-V600E melanoma cell lines drug sensitive vs drug resistant |
|
