|
| Status |
Public on Mar 12, 2015 |
| Title |
KC2 (3) |
| Sample type |
RNA |
| |
|
| Source name |
KC2 cell line, highly metastatic cells
|
| Organism |
Mus musculus |
| Characteristics |
cell line: KC2
strain/background: 127s
genotype/variation: K-rasLA1/+; Cdkn1a-/-
cell type: lung adenocarcinoma cell line
metastasis level: highly metastatic
|
| Growth protocol |
K-rasLA1/+ mice were crossed with Cdkn1a+/+ and Cdkn1a-/- mice to generate K-rasLA1/+; Cdkn1a+/+ and K-rasLA1/+; Cdkn1a-/- mice, respectively. Mice were of 127s background. Murine lung adenocarcinoma cell lines (KC1-KC4) were derived from 6 different primary lung tumors obtained at necropsy from 4 different KC mice using the Cancer Cell Isolation Kit (Affymetrix) and cultured in RPMI 1640 medium supplemented with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
According to Affymetrix protocol.
|
| Label |
biotin
|
| Label protocol |
According to Affymetrix protocol.
|
| |
|
| Hybridization protocol |
According to Affymetrix protocol.
|
| Scan protocol |
According to Affymetrix protocol.
|
| Description |
KC2 lung cancer cell line, highly metastatic cells (biological replicate 3).
|
| Data processing |
Gene expression values were processed by Asuragen (Austin, TX) and subjected to quantile normalization.
|
| |
|
| Submission date |
Jul 29, 2013 |
| Last update date |
Mar 12, 2015 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE49315 |
Gene expression profiling of metastatic lung cancer cell lines |
|
