|
| Status |
Public on Sep 01, 2013 |
| Title |
R1-AD1 + DHT 3 |
| Sample type |
RNA |
| |
|
| Source name |
R1-AD1 prostate cancer cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: R1-AD1 prostate cancer cells
genotype: Full length AR
knockdown: siCNTL
treatment: 1nM DHT
|
| Treatment protocol |
Cells were electroporated with either siCNTL or siAR-exon1. R1-AD1 cells were electroporated with siCNTL. Electroporated cells were plated in RPMI 1640 medium supplemented with 10% CSS. After 48h, cells were switched to serum free RPMI-1640 medium supplemented with 1nM DHT or 0.1% (v/v) ethanol (vehicle control) for 24 hours.
|
| Growth protocol |
Cells were grown in RPMI-1640 with 10% FBS on tissue culture treated plastic.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol and column purified using Qiagen RNA purification columns.
|
| Label |
biotin
|
| Label protocol |
Total RNA was converted to amplified, biotinylated, antisense cRNA using the Illumina TotalPrep-96 RNA Amplification Kit (Life Technologies) in accordance with the manufacturer's specifications.
|
| |
|
| Hybridization protocol |
150ng of biotin-labeled cRNA was hybridized with Illumina Beadchips using the HumanHT-12 v4 Expression Beadchip Kit in accordance with the manufacturer's specifications
|
| Scan protocol |
Hybridized Beadchips were scanned with an Illumina iScan.
|
| Data processing |
Average probe intensity data was extracted from iScan image files using the Partek Report Plug-in for Illumina GenomeStudio and then imported to Partek Genomics Suite 6.6. Raw intensity data was log2 transformed and quantile normalized using Partek GS 6.6.
|
| |
|
| Submission date |
Jul 25, 2013 |
| Last update date |
Sep 01, 2013 |
| Contact name |
Michael David Nyquist |
| E-mail(s) |
micknyquist@gmail.com
|
| Phone |
612-961-6367
|
| Organization name |
University of Minnesota
|
| Department |
Genetics Cell Developmental Bio
|
| Lab |
Scott Dehm and Dan Voytas
|
| Street address |
Masonic Cancer Center, University of Minnesota, Mayo Mail Code 806 420 Delaware St SE
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL10904 |
| Series (1) |
| GSE49196 |
TALEN-Engineered AR Gene Rearrangements Reveal Endocrine Uncoupling of Androgen Receptor in Prostate Cancer |
|
