|
| Status |
Public on Mar 01, 2014 |
| Title |
DMSO treatment, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
DMSO-treated MCF7 cells, 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
treatment: DMSO-treated for 24 hours
phenotype: tamoxifen-resistant
|
| Treatment protocol |
tamoxifen resistant MCF7 cells were maintained in phenol red-free medium containing 10% charcoal-dextran-stripped FCS and 100 nM of 4-HT. JQ1 or DMSO was added and incubated for 24 hours before harvest for RNA purification.
|
| Growth protocol |
tamoxifen-resistant MCF7 cells were cultured in phenol red-free medium containing 10% charcoal-dextran-stripped FCS and 100 nM of 4-HT.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy kit was used for total RNA extraction according to the manufacturer's instructions.
|
| Label |
biotinylated
|
| Label protocol |
cDNA were prepared according to the standard Affymetrix protocol for whole transcriptome analysis from 100ng total RNA.
|
| |
|
| Hybridization protocol |
Following end-labeling with biotin, 2 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix 3000 scanner with 7G upgrade
|
| Description |
gene expression data from DMSO-treated MCF7 cells, 24 hours
|
| Data processing |
The data were analyzed with Affymetrix Expression Console using Affymetrix default analysis settings and RMA as normalization method on core dataset.
|
| |
|
| Submission date |
Jul 23, 2013 |
| Last update date |
Mar 01, 2014 |
| Contact name |
Qin Feng |
| E-mail(s) |
qfeng4@central.uh.edu
|
| Phone |
8328428824
|
| Organization name |
University of Houston
|
| Department |
Biology and Biochemistry
|
| Street address |
3517 Cullen Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77204 |
| Country |
USA |
| |
|
| Platform ID |
GPL10739 |
| Series (1) |
| GSE49124 |
Expression data from JQ1 (0.2 uM) treated tamoxifen-resistant MCF7 cells |
|
