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Status
Public on Jul 17, 2024
Title
NU22
Sample type
RNA
Source name
Homo Sapiens
Organism
Homo sapiens
Characteristics
sample type: Normal Ureter
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and the RNAprotect reagent (Qiagen) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen). RNA quality and concentration was determined by analysis with an Agilent 2100 bioanalyzer at the Harvard-Partners Center for Genetics and Genomics (Harvard Medical School, Cambridge, MA USA).
Label
Cy3
Label protocol
Cy3 9mer Wobble TriLink, Biotechnologies, 50 O.D., N46-0001-50
Hybridization protocol
MAUI Hybridization System, BioMicro Systems, 02-A002-03
Scan protocol
DNA Microarray Scanner, Agilent, G2565B
Data processing
Raw values were trimmed to exclude 4295 random probes. Raw values were log2-transformed, and samples were median centered. The median-centered log2 values were then quantile-normalized in R (normalize.quantiles).
Submission date
Jul 18, 2013
Last update date
Jul 17, 2024
Contact name
Bogdan Czerniak
E-mail(s)
bczernia@mdanderson.org
Organization name
UT MD Anderson Cancer Center
Department
Pathology
Street address
1515 Holcombe
City
Houston
State/province
TX
ZIP/Postal code
77030
Country
USA
Platform ID
GPL17471
Series (1)
GSE49024
Expression analysis of RB1 region in bladder cancer cases
Data table header descriptions
ID_REF
VALUE
quantile-normalized, median-centered, log2 transformed raw intensity values.
Data table
ID_REF
VALUE
CHR13FS045100002
-0.389118602
CHR13FS045100011
0.025887274
CHR13FS045100019
-0.015221237
CHR13FS045100027
-0.141842559
CHR13FS045100038
-0.088870571
CHR13FS045100047
0.189634082
CHR13FS045100054
-0.125084695
CHR13FS045100065
-0.276347417
CHR13FS045100074
-0.229429319
CHR13FS045100083
-0.16706075
CHR13FS045100091
-0.357721759
CHR13FS045100099
-0.350645474
CHR13FS045100108
-0.003518627
CHR13FS045100117
0.025559078
CHR13FS045100126
0.057239519
CHR13FS045100135
0.266937491
CHR13FS045100145
-0.015940183
CHR13FS045100189
0.007966085
CHR13FS045100198
-0.197433262
CHR13FS045100206
-0.329807346
Total number of rows: 385012 Table truncated, full table size 11052 Kbytes .Supplementary file
Size
Download
File type/resource
GSM1192522_10109402.csv.gz
5.2 Mb
(ftp) (http)
CSV
GSM1192522_10109402_532.pair.gz
5.7 Mb
(ftp) (http)
PAIR
Processed data included within Sample table
Processed data provided as supplementary file