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Sample GSM1178275 Query DataSets for GSM1178275
Status Public on Jul 01, 2013
Title K-562_originating cell line_R2
Sample type RNA
 
Source name K-562, originating cell line
Organism Homo sapiens
Characteristics originating cell line: K-562
tumor type: chronic myelogenous (blast crisis) leukemia
in vivo passage: P0
Treatment protocol N/A
Growth protocol All cell lines were obtained from the Division of Cancer Treatment and Diagnosis Tumor Repository (DTP, FNLCR, Frederick, MD) or American Type Culture Collection (ATCC) (Manassas, Va). In addition, the identities of all cell lines used in this study were confirmed using Identifiler® STR genotyping (Applied Biosystems). All mice used in the study were obtained from the Animal Production Program (FNLCR, Frederick, MD). Tumor cells for inoculation were derived from the 4th in vitro passage from cryopreserved cell stocks. Cells (1 x 10^7 cells/0.1 ml/inoculation) were inoculated subcutaneously into mice (n=10 or more mice depending on the tumor cell line). For comparative purposes, 2-3 samples of in vitro cultivated cells at passage 4 post-cryopreservation were also prepared for microarray analysis (P0). When tumors at P1, P4 and P10 reached approximately 500 mg (10x10 mm), 5 tumors were harvested, cut into small pieces and flash frozen in liquid nitrogen using pre-chilled tubes. Samples were transferred to a -70°C freezer for holding prior to processing. Serial tumor passage was achieved by harvesting donor tumor material (n=2 to 5 donors) at each passage, mincing the tumors (2x2 mm fragments), pooling the samples and inoculating the next recipients subcutaneously using tumor implant trocars. Athymic nude mice (nu/nu NCr) served as the primary host for most xenograft studies. In several instances (particularly with human leukemias and lymphomas) it was difficult to establish and passage tumors in nu/nu NCr mice. In these instances, SCID.NCr or NOD.SCID/NCr mice were assessed for suitability. In general, female mice were used as they are less aggressive, easily group-housed and routinely used in xenograft experiments. Male mice were used for prostate tumors (PC-3, PC-3/M). Finally, in those instances where hormone effects on tumor growth are important (e.g., estradiol-dependent breast), mice were supplemented with estradiol.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using RNeasy (Qiagen).
Label Biotin
Label protocol The RNA samples were submitted to Expression Analysis, Inc. (Durham, NC) for microarray analysis using the GeneChip® Human U133 Plus 2.0 Array (Affymetrix, Santa Clara, CA). Samples were labeled according to the "Affymetrix Technical Manual" (www.expressionanalysis.com).
 
Hybridization protocol Samples were hybridized according to the "Affymetrix Technical Manual" (www.expressionanalysis.com).
Scan protocol Microarray images were captured using a GeneChip® 3000 Scanner.
Description K-562 P0 T2
Gene expression data from K-562 originating cell line.
Data processing Affymetrix Expression Console v1.3.
 
Submission date Jun 30, 2013
Last update date Jul 01, 2013
Contact name Luke Hunter Stockwin
E-mail(s) stockwinl@mail.nih.gov
Phone 301-846-5431
Organization name NCI Frederick
Department DTP
Street address Ft. Detrick
City Frederick
State/province MD
ZIP/Postal code 21701
Country USA
 
Platform ID GPL570
Series (1)
GSE48433 Microarray analysis of xenograft models in use at the Developmental Therapeutics Program of the National Cancer Institute (DTP-NCI)

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 597.279 P 0.000753643
AFFX-BioB-M_at 706.562 P 8.14279e-05
AFFX-BioB-3_at 551.94 P 0.000126798
AFFX-BioC-5_at 1424.65 P 7.00668e-05
AFFX-BioC-3_at 2542.35 P 4.42873e-05
AFFX-BioDn-5_at 4092.78 P 4.42873e-05
AFFX-BioDn-3_at 8267.19 P 0.000169227
AFFX-CreX-5_at 20428.2 P 5.16732e-05
AFFX-CreX-3_at 19636.2 P 4.42873e-05
AFFX-DapX-5_at 19.3663 A 0.139482
AFFX-DapX-M_at 39.0962 A 0.250796
AFFX-DapX-3_at 2.85401 A 0.994435
AFFX-LysX-5_at 11.4272 A 0.39692
AFFX-LysX-M_at 4.00976 A 0.897835
AFFX-LysX-3_at 4.90414 A 0.544587
AFFX-PheX-5_at 2.58087 A 0.921998
AFFX-PheX-M_at 4.0407 A 0.9273
AFFX-PheX-3_at 28.8568 A 0.58862
AFFX-ThrX-5_at 9.4972 A 0.737173
AFFX-ThrX-M_at 21.003 A 0.340661

Total number of rows: 54675

Table truncated, full table size 1629 Kbytes.




Supplementary file Size Download File type/resource
GSM1178275_EA06017_129740_H133+_125.CEL.gz 4.7 Mb (ftp)(http) CEL
GSM1178275_EA06017_129740_H133+_125.CHP.gz 491.1 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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