|
| Status |
Public on Aug 08, 2013 |
| Title |
genomic DNA from liver sample 33 |
| Sample type |
genomic |
| |
|
| Source name |
liver tissue 33
|
| Organism |
Homo sapiens |
| Characteristics |
group: Healthy Obese
age: 51
sex (1 - male, 2 - female): 2
bariatric surgery (1 - before surgery, 2 - after): 2
bmi: 29.0
tissue: liver
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
For homogenisation of five to ten milligram frozen tissue and subsequent nucleic acid isolation, tubes with 1.4 mm ceramic beads (Precellys, Villeurbanne, France) and the AllPrep DNA/RNA Mini Kit (QIAGEN, Hilden, Germany) were used.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using iScan Reader using standard recommended Illumina scanner setting
|
| Description |
Healthy Obese
|
| Data processing |
GenomeStudio V2011.1; methylation module 1.9.0
|
| |
|
| Submission date |
Jun 26, 2013 |
| Last update date |
Aug 08, 2013 |
| Contact name |
Ole Ammerpohl |
| Organization name |
UK Ulm
|
| Street address |
Albert-Einstein-Allee 11
|
| City |
Ulm |
| ZIP/Postal code |
89081 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE48325 |
DNA methylation analysis in non-alcoholic fatty liver disease suggests distinct disease-specific and remodeling signatures after bariatric surgery |
|
