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Sample GSM1169052 Query DataSets for GSM1169052
Status Public on Aug 21, 2013
Title Antibiotics-Distal 85
Sample type RNA
 
Source name Distal intestine, antibiotics
Organism Sus scrofa
Characteristics tissue: distal intestine
treatment: Antibiotics were given twice daily from birth to tissue collection at d 5.
Treatment protocol Piglets from each litter were stratified according to birth weight and sex, and allocated into controls (CON, n=13) and an intervention group receiving oral and systemic broad-spectrum antibiotics (Ampicillin, Gentamycin, Metronidazol, ANTI, n=11). To assure high systemic and intra luminal MIC values antibiotics were given both orally and intramuscularly. All antibiotics were given directly after feeding with an oral bolus and control pigs were given corresponding amounts of saline. On day five, all piglets were euthanized, and small intestinal tissue collected.
Growth protocol Twenty-four preterm piglets (Landrace x Large white x Duroc) were delivered by caesarean section on day 105 of gestation from two healthy sows. Piglets were housed in incubators with regulated temperature and oxygen supply. All piglets were initially provided with parenteral nutrition via a vascular catheter, combined with small amounts of minimal enteral nutrition. On day three, all parenteral nutrition was stopped and total enteral nutrition was given through an oro-gastric feeding tube until sacrifice and tissue collection on day five.
Extracted molecule total RNA
Extraction protocol Tissue was lyzed in Trizol (Invitrogen). RNA was isolated using standard chloroform/isopropanol steps. Total RNA was further purified with the RNeasy Kit (Qiagen), including an RNase-free DNase I (Qiagen) digestion step.
Label biotin
Label protocol Fragmented and biotin-labelled aRNA was synthesized from total RNA using the 3' IVT Express Kit (Affymetrix) according to the manufacturer's protocol.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
Description Caesarean-delivered preterm pigs were fed 3 d of parenteral nutrition followed by 2 d of enteral formula feeding. Antibiotics (n=11) or control saline (n=13) were given twice daily from birth to tissue collection at d 5. NEC-lesions and intestinal structure, function, microbiology and immunity markers were recorded.
Data processing Initial data analysis was performed using Affymetrix GCOS 1.2 software. Data were further analyzed using Bioconductor (http://www.bioconductor.org) from the R Project for Statistical Computing (http://www.r-project.org). Normalization was performed with gcRMA. The moderated t-test provided by the Bioconductor package limma was used to test for differential expression. Data was considered significant when P<0.01, FC>2.
 
Submission date Jun 19, 2013
Last update date Aug 21, 2013
Contact name Denise Kelly
E-mail(s) D.Kelly@rri.sari.ac.uk
Phone +44 1224 716648
Organization name University of Aberdeen
Lab Gut Immunology Group
Street address Institute of Medical Sciences
City Aberdeen
ZIP/Postal code AB25 2ZD
Country United Kingdom
 
Platform ID GPL3533
Series (1)
GSE48125 Neonatal antibotic prophylaxis modulates intestinal immunity and prevents necrotizing enterocolitis in preterm neonates

Data table header descriptions
ID_REF
VALUE gcRMA normalized signal intensity, log2 scaled

Data table
ID_REF VALUE
AFFX-BioB-3_at 8.903995491
AFFX-BioB-5_at 8.967647955
AFFX-BioB-M_at 9.432540809
AFFX-BioC-3_at 10.80968427
AFFX-BioC-5_at 9.745331073
AFFX-BioDn-3_at 12.35821244
AFFX-BioDn-5_at 12.09684041
AFFX-CreX-3_at 15.09765623
AFFX-CreX-5_at 15.07074869
AFFX-DapX-3_at 12.15054538
AFFX-DapX-5_at 7.102990859
AFFX-DapX-M_at 10.66725308
AFFX-LysX-3_at 8.671652272
AFFX-LysX-5_at 6.770352765
AFFX-LysX-M_at 6.360949766
AFFX-PheX-3_at 8.440421695
AFFX-PheX-5_at 6.795087319
AFFX-PheX-M_at 7.694272213
AFFX-r2-Bs-dap-3_at 12.57582671
AFFX-r2-Bs-dap-5_at 8.492876569

Total number of rows: 24123

Table truncated, full table size 674 Kbytes.




Supplementary file Size Download File type/resource
GSM1169052_IM_140812_85_Porcine_.CEL.gz 2.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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