|
| Status |
Public on Jun 30, 2013 |
| Title |
WT MEFs at passage 3_biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: MEFs
genotype/variation: wild type
|
| Treatment protocol |
MEFs were isolated from E13.5 embryos and passaged 3 times prior to experiment plating and collection.
|
| Growth protocol |
MEFs cells were passaged in DMEM supplemented with 10% FBS and pen/strep.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from MEFs using RNeasy Kits (Qiagen) according to manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA. RNA was reverse transcribed with T7-oligo(dT) primer and biotin-labeled
|
| |
|
| Hybridization protocol |
Four replicates of each group were prepared, labeled, and hybridized to Affymetrix mouse 430 2.0 GeneChip
|
| Scan protocol |
GeneChips were scanned on Affymetrix GeneChip scanner 3000
|
| Description |
WT_MEF1.CEL
|
| Data processing |
Following QC checks, microarray probe level data were normalized, background adjusted, and summarized with the RMA algorithm (Robust Multichip Analysis)
|
| |
|
| Submission date |
Jun 10, 2013 |
| Last update date |
Jun 30, 2013 |
| Contact name |
Peter F Johnson |
| E-mail(s) |
johnsope@mail.nih.gov
|
| Phone |
301 846-1627
|
| Organization name |
National Cancer Institute
|
| Department |
Center for Cancer Research
|
| Lab |
Mouse Cancer Genetics Program
|
| Street address |
1050 Boyles St
|
| City |
FREDERICK |
| State/province |
MD |
| ZIP/Postal code |
21702-1201 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE47777 |
Whole genome expression data comparison between WT and Cebpg-/- MEFs. |
|
