|
| Status |
Public on Jun 22, 2006 |
| Title |
Control_biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
ATCC CRL 1582 (lymphoblast)
|
| Organism |
Homo sapiens |
| Characteristics |
male 19 years old
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
For this purpose the cells were cultured and sub-cultured bi- weekly in RPMI-1640 medium containing fetal calf serum (10%), penicillin (100 U/ml), streptomycin (100 µg/ml) and L-glutamine (2 mM) and under humidified air containing 5% CO2 and at 37?C. The cultures were tested routinely for contamination by mycoplasma. The cells were cultured in drug-free-medium for at least 3 passages prior to harvesting during logarithmic phase growth (at a density of approximately 0.8-1.5x106/ml) for experimental studies. A Coulter Multi-sizer (Coulter Electronics, Luton, United Kingdom) was used to count the cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Midi kit in accordance with manufacturer’s instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip human U133A Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from untreated MOLT4 cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jun 21, 2006 |
| Last update date |
Jun 21, 2006 |
| Contact name |
Ali Moshfegh |
| E-mail(s) |
ali.moshfegh@ki.se
|
| Organization name |
Karolinska Institutet
|
| Department |
Oncology and Pathology
|
| Street address |
Z5:01
|
| City |
Stockholm |
| ZIP/Postal code |
17176 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE5118 |
Expression data from MOLT4 human cell line |
|
