|
| Status |
Public on Dec 31, 2014 |
| Title |
IMSC#3, 21 days adipocyte differentiation, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Immortalized human bone marrow-derived mesenchymal stromal cell line IMSC#3, 21 days adipocyte differentiation, replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: IMSC#3
sample origin: Human bone marrow-derived mesenchymal stromal cell
gender: Male
|
| Growth protocol |
Cells were seeded in 48- and 6-well plates (Nunc) at 25,000 and 100,000 cells per well, respectively. Next day, the medium was replaced with adipocyte induction medium containing 1 µM Dexamethasone (Decadron), 0.5 mM iso-butyl-methyl-xanthine and 50 µM Indomethacine (both from Sigma-Aldrich). The differentiation medium was replaced twice a week for 21 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the standard TRIzol procedure (Invitrogen), and further purified with an RNeasy mini column (Qiagen GmbH, Hilden, Germany), according to the manufacturers’ instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data was quantile normalisation in GeneSpring GX10 (Agilent)
|
| |
|
| Submission date |
Jun 05, 2013 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Leonardo A. Meza-Zepeda |
| E-mail(s) |
L.A.Meza-Zepeda@ous-research.no
|
| Phone |
+47-99035706
|
| Organization name |
Oslo University Hospital
|
| Department |
Department of Tumor Biology
|
| Lab |
Meza-Zepeda
|
| Street address |
Ullernchausseen 70
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL6884 |
| Series (2) |
| GSE47647 |
Generation and Characterization of an immortalized human mesenchymal stromal cell line model [Illumina adipocyte differentiation] |
| GSE47651 |
Generation and Characterization of an immortalized human mesenchymal stromal cell line model |
|
