|
| Status |
Public on Jun 20, 2006 |
| Title |
Breast epithelial cells derived from fibroadenoma MCF-10F, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
breast epithelial cells derived from fibroadenoma
|
| Organism |
Homo sapiens |
| Characteristics |
Genotype: slightly aneuploid
|
| Treatment protocol |
Cells were grown in medium in normal condition without 17-beta-estradiol treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 8 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from nontumorigenic ER(-) human breast epithelial cells
|
| Data processing |
The data were analyzed with dChip softwareusing invariant-set normalization method.
|
| |
|
| Submission date |
Jun 19, 2006 |
| Last update date |
Jun 19, 2006 |
| Contact name |
Yong Huang |
| E-mail(s) |
yh9fj@virginia.edu
|
| Phone |
(434) 243-0842
|
| Organization name |
University of Viginia
|
| Department |
Medicine
|
| Street address |
1340 Jefferson Park Ave
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE5102 |
Genomic profile of the estrogen induced neoplastic transformation of the human breast epithelial cell MCF10F |
|
