|
Status |
Public on Jul 15, 2013 |
Title |
Testis A |
Sample type |
RNA |
|
|
Source name |
WT C57BL/6 adult Male
|
Organism |
Mus musculus |
Characteristics |
marker: CD34+ Ve-Cad+ IgG- strain: C57BL/6 cell type: Testicle Endothelial Cells gender: male
|
Treatment protocol |
NA
|
Growth protocol |
NA
|
Extracted molecule |
total RNA |
Extraction protocol |
PicoPure RNA isolation kit
|
Label |
Biotin
|
Label protocol |
Nugen Ovation Amplification, Ovation Exon Module, Encore Biotin Module for preparation, fragmentation, and labeling.
|
|
|
Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 45° for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 200μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 16 hours at 45° at 60rpm• Fluidics washing is FS450_0007
|
Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
Description |
Testicle Endothelial Cells
|
Data processing |
All Data was processed using GeneSpring 11 software with RMA normalization MoGene-1_0-st-v1, HuGene-1_0-st-v1 MoGene-1_0-st-v1-r3, HuGene-1_0-st-v1-r3
|
|
|
Submission date |
May 17, 2013 |
Last update date |
Jul 15, 2013 |
Contact name |
Daniel J Nolan |
E-mail(s) |
dnolan@angiocrinebio.com
|
Organization name |
Weill Cornell Medical College
|
Department |
Genetic Medicine
|
Lab |
Rafii
|
Street address |
1300 York Ave
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE47067 |
In Vivo Endothelial Cell Heterogeneity |
|