|
| Status |
Public on Jul 15, 2013 |
| Title |
Testis A |
| Sample type |
RNA |
| |
|
| Source name |
WT C57BL/6 adult Male
|
| Organism |
Mus musculus |
| Characteristics |
marker: CD34+ Ve-Cad+ IgG-
strain: C57BL/6
cell type: Testicle Endothelial Cells
gender: male
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PicoPure RNA isolation kit
|
| Label |
Biotin
|
| Label protocol |
Nugen Ovation Amplification, Ovation Exon Module, Encore Biotin Module for preparation, fragmentation, and labeling.
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 45° for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 200μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 16 hours at 45° at 60rpm• Fluidics washing is FS450_0007
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
Testicle Endothelial Cells
|
| Data processing |
All Data was processed using GeneSpring 11 software with RMA normalization
MoGene-1_0-st-v1, HuGene-1_0-st-v1
MoGene-1_0-st-v1-r3, HuGene-1_0-st-v1-r3
|
| |
|
| Submission date |
May 17, 2013 |
| Last update date |
Jul 15, 2013 |
| Contact name |
Daniel J Nolan |
| E-mail(s) |
dnolan@angiocrinebio.com
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Genetic Medicine
|
| Lab |
Rafii
|
| Street address |
1300 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE47067 |
In Vivo Endothelial Cell Heterogeneity |
|
