|
| Status |
Public on Jun 01, 2013 |
| Title |
Splenic B cells_tripl_2 |
| Sample type |
RNA |
| |
|
| Source name |
mature, CD43 negative splenic B cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Resting B cell
strain: C57Bl/6 x FVB
|
| Treatment protocol |
RNA was extracted after one PBS wash
|
| Growth protocol |
Resting (B and T) cells (Day 0) were isolated directly from the spleen;Activated (B and T) cells (Day 4) were harvested after a four day in vitro activation assay
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol was used to extract total RNA according to manufacturers instructions
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled using the Affymetrix GeneChip® WT (Whole Transcript) Terminal Labeling Kit, according to manufacturers instructions
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on the mouse gene 1.0st array in a GeneChip Hyb oven 640 according to manufacturer instructions. Washing was done in a GeneChip WS-system according to manufacturer instructions.
|
| Scan protocol |
Scanning was done on a geneChip scanner 7G incl. autocaroussel using affymetrix launcher software for downstream image processing
|
| Description |
Gene expression data for resting B cells
|
| Data processing |
Normalized gene expression values were calculated using robust multi-array average (RMA)
|
| |
|
| Submission date |
May 13, 2013 |
| Last update date |
Jun 01, 2013 |
| Contact name |
sjoerd holwerda |
| E-mail(s) |
s.holwerda@hubrecht.eu
|
| Organization name |
Hubrecht Institute
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| ZIP/Postal code |
3584 CT |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE46865 |
Expression data for resting and activated B and T cells |
| GSE47129 |
Allele specific analysis of the immunoglobulin heavy chain locus |
|
