|
| Status |
Public on May 22, 2014 |
| Title |
MLL#20 |
| Sample type |
genomic |
| |
|
| Source name |
Leukemic cells obtained from bone marrow or blood at diagnosis
|
| Organism |
Homo sapiens |
| Characteristics |
disease: leukemia
cell type: de novo AML
|
| Treatment protocol |
None
|
| Growth protocol |
DNA was extracted from cryopreserved mononuclear cell preparations of diagnostic bone marrow or peripheral blood from AML cases with t(9;11)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
AllPrep DNA/RNA Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
250 nanograms of genomic DNA were digested to completion with NspI or StyI, ligated to adapaters and amplified by PCR. 200-270 micrograms of pooled amplified DNA were fragmented and end-labeled with a biotinylated nucleotide according to the Genome-Wide Human SNP Nsp/Sty 6.0 User Guide 702504 Rev 3, 2008, Affymetrix)
|
| |
|
| Hybridization protocol |
200-270 micrograms of amplified and biotinylated DNA were hybridized for 16-18 hr at 50C on a GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
|
| Description |
DNA extracted from tumor cells obtained at diagnosis
|
| Data processing |
Genotyping Console/birdseed-v2
|
| |
|
| Submission date |
May 08, 2013 |
| Last update date |
May 22, 2014 |
| Contact name |
Michael Kuehn |
| E-mail(s) |
michael.kuehn@uniklinik-ulm.de
|
| Organization name |
University Hospital of Ulm
|
| Street address |
Albert-Einstein-Allee 23
|
| City |
Ulm |
| ZIP/Postal code |
89081 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE46745 |
Genome-wide Genotyping of Acute Myeloid Leukemia with Translocation t(9;11)(p22;q23) Reveals Novel Recurrent Genomic Alterations |
|
