|
| Status |
Public on May 08, 2013 |
| Title |
JIKK2 |
| Sample type |
RNA |
| |
|
| Source name |
JIKK2_2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Huh7.5.1
treatment: treated with IKKalpha siRNA and subsequently infected with HCV
|
| Treatment protocol |
Cells were treated with either non-targeting control siRNA or siRNA against IKKalpha at a final concentration of 50 nM. After 72 h, cells were either mock infected or infected with HCV JFH-1 strain with the M.O.I. of 0.5. At 48 h post-infection, cells were harvested, and total cellular RNA was extracted using a QIAGEN RNeasy mini kit. Total RNA was quantified, measured for purity, and then subjected to microarray.
|
| Growth protocol |
All cell lines were growed with/ with no the HCV infection as in the reference.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
RMA by software Partek v6.5
|
| |
|
| Submission date |
Apr 30, 2013 |
| Last update date |
May 08, 2013 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE46528 |
Hepatitis C Virus Infection Activates a Novel Innate Pathway Involving IKKα in Lipogenesis and Viral Assembly |
|
