|
| Status |
Public on Apr 30, 2013 |
| Title |
RAL318_8hr_drug |
| Sample type |
RNA |
| |
|
| Source name |
Whole_fly_RAL318_8hr_drug
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: whole fly
Sex: male
age: 3-6 days
line: RAL318
time: 8 hr
treatment: tunicamycin
|
| Treatment protocol |
Flies were placed on Tunicamycin or control food for 8 or 20 hours. Food: 1% sucrose, 1.3% low melting agarose, water. Tunicamycin concentration: 12uM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by standard phenol/chloroform protocol
|
| Label |
Cy3
|
| Label protocol |
Samples were labelled following the Ambion amino allyl messageAMP II aRNA Kit
|
| |
|
| Hybridization protocol |
Hybridized overnight at 60 C
|
| Scan protocol |
Microarrays were scanned on an Axon 4000B scanner
|
| Description |
gene expression after 8 hr on tunicamycin
|
| Data processing |
Agilent Feature Extraction and GenePix Pro-6.0 software
|
| |
|
| Submission date |
Apr 26, 2013 |
| Last update date |
Apr 30, 2013 |
| Contact name |
Clement Y Chow |
| E-mail(s) |
clementychow@gmail.com
|
| Phone |
6072551707
|
| Organization name |
Cornell University
|
| Street address |
227 Biotech Bldg
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853-2703 |
| Country |
USA |
| |
|
| Platform ID |
GPL17080 |
| Series (1) |
| GSE46425 |
Using natural variation Drosophila to discover previously unknown ER stress genes |
|
