|
Status |
Public on Jun 14, 2013 |
Title |
Control_+DEX_rep2 |
Sample type |
RNA |
|
|
Source name |
Erythroid cells
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 age: E14.5 tissue: fetal liver cell type: early erythroid progenitors infection: control virus culture conditions: +DEX
|
Treatment protocol |
Early erythroid progenitors were infected with control virus or viruses encoding RBP shRNAs, and cultured in medium for 3 days before mRNA extraction.
|
Growth protocol |
Early erythroid progenitors were enriched from mouse E14.5 fetal liver. The first step includes a negative selection for erythroid progenitors by magnetic depleting of mature blood cells (lineage positive cells), early multipotent GEMM colony forming cells (Sca-1 positive cells), and myeloid colony-forming cells (CFU-G/M/Mk) (CD16/32 positive, CD41 positive, or CD34 positive cells). The second step includes a FACS based positive selection of early erythroid progenitor process (c-kit positive and CD71 10%low cells).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNAs were extracted by using Qiagen RNA extraction kit following the manufacturer's instruction.
|
Label |
Cy3
|
Label protocol |
Label was performed following one-color microarray-based gene expression analysis low input quick amp labeling protocol (Agilent Technologies 'one-color microarray-based gene expression analysis').
|
|
|
Hybridization protocol |
Agilent expression arrays were hybridized according to manufacturer instructions and scanned using an Agilent DNA microarray scanner.
|
Scan protocol |
Arrays were scanned using an Agilent DNA microarray scanner with SureScan high-resolution technology.
|
Description |
mRNA expression in day3 cultured cells (+DEX) infected with control virus repeat 2
|
Data processing |
Data was extracted from the array images using Agilent’s Feature Extraction software, quantile normalized using limma from Bioconductor in R, and processed.
|
|
|
Submission date |
Apr 19, 2013 |
Last update date |
Jun 14, 2013 |
Contact name |
Lingbo Zhang |
E-mail(s) |
zhanglb@wi.mit.edu
|
Organization name |
Whitehead Institute for Biomedical Research and MIT Department of Biology
|
Street address |
Nine Cambridge Center
|
City |
Cambridge |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platform ID |
GPL10333 |
Series (2) |
GSE46215 |
Identification of genes regulated by glucocorticoids and an RNA binding protein (RBP) in early erythroid progenitor using microarray experiment |
GSE46216 |
Role of glucocorticoids and an RNA binding protein (RBP) in early erythroid progenitors |
|