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Sample GSM1123755 Query DataSets for GSM1123755
Status Public on Oct 14, 2014
Title Breast Cancer Sample BC1904
Sample type RNA
 
Source name primary breast cancer tissue
Organism Homo sapiens
Characteristics gender: female
tissue: pretreatment excision biopsy
cell type: invasive mammary carcinoma
Treatment protocol samples were snap frozen and stored at -80°C
Extracted molecule total RNA
Extraction protocol RNeasy kit, Qiagen. according to the manufacturer's instructions
Label biotin
Label protocol Starting from 5 µg total RNA, labeled cRNA was prepared using the Roche Microarray cDNA Synthesis, Microarray RNA Target Synthesis (T7) and Microarray Target Purification Kit, according to the manufacturers instructions
 
Hybridization protocol Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45°C on GeneChip HG-U133a Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from primary breast tumors
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Apr 16, 2013
Last update date Oct 14, 2014
Contact name Thomas Karn
E-mail(s) t.karn@em.uni-frankfurt.de
Organization name J.W.Goethe-University
Street address Theodor-Stern-Kai 7
City Frankfurt
ZIP/Postal code 60590
Country Germany
 
Platform ID GPL96
Series (1)
GSE4611 Breast Cancer Gene Expression Data from Frankfurt Series

Data table header descriptions
ID_REF
VALUE MAS5

Data table
ID_REF VALUE
1007_s_at 3925.9
1053_at 344.5
117_at 310.2
121_at 1350
1255_g_at 86
1294_at 460.6
1316_at 145.5
1320_at 38.1
1405_i_at 147.1
1431_at 53.9
1438_at 338.1
1487_at 706.8
1494_f_at 359.9
1598_g_at 2518.8
160020_at 1725.8
1729_at 628.4
177_at 163.2
1773_at 171.4
179_at 1418.5
1861_at 472.1

Total number of rows: 22283

Table truncated, full table size 360 Kbytes.




Supplementary file Size Download File type/resource
GSM1123755_BC1904wdh.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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