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Sample GSM1122226 Query DataSets for GSM1122226
Status Public on Apr 26, 2013
Title 293_AMO-CTRL_rep5
Sample type RNA
 
Source name Flp-in T-REx 293-PTH-AGO1 AMO-CTRL treated
Organism Homo sapiens
Characteristics treatment: control inhibitor
cell line: Flp-in T-REx 293-PTH-AGO1
Treatment protocol Flp-In T-REx 293-PTH-AGO1 cells were transfected with Lipofectamine 2000 (Life Technologies) and miR-92a (mA/ZEN/mCmAmGmGmCmCmGmGmGmAmCmAmAmGmUmGmCmA mAmU/ZEN/mA; IDT; 6.25nM) or control inhibitor (mC/ZEN/mGmCmGmAmCmUmAmUmAmCmGmCmGmCmAmAmUmAmUmGmG/ZEN/mU; IDT; 6.25nM)
Growth protocol Flp-In T-REx 293 cells were stably transfected with a plasmid encoding AGO1 N-terminally tagged with ProteinA-TEV protease cleavage site-Hisx6. Expression of tagged AGO1 is tetracycline inducible. For the purpose of this microarray analysis AGO1 expression was not induced. Cells were grown in Dulbecco’s modified Eagle medium (DMEM) with high glucose, supplemented with 10% fetal bovine serum at 37°C under humidified air with 5% CO2
Extracted molecule total RNA
Extraction protocol total RNA was izolated 48 hours post transfecton with TRIZOL (Life Technologies) according to manufacturers instructions; sample integrity was confirmed on Bioanalyzer and samples were further processed by the Source BioScience UK Limited
Label biotin
Label protocol sense strand cDNA was prepared with Ambion WT Expression Kit (Life Technologies, 4411973), fragmentation and biotin labelling was done with GeneChip WT terminal labelling kit (Affymetrix, 900670)
 
Hybridization protocol fragmented and labelled cDNA was hybridized to GeneChip® Human Exon 1.0 ST (Affymetrix,900694) at 45°C for 16 hours, washing and staining was done using GeneChipHybridization Wash and Stain kit (Affymetrix, 900720)
Scan protocol hybridized microarrays were scanned on GeneChip Scanner 3000 (Affymetrix, P7N 00-00212)
Data processing RMA-EXON-CORE-DABG protocol
HuEx-1_0-st-v2.r2.pgf
HuEx-1_0-st-v2.r2.dt1.hg18.core.mps
 
Submission date Apr 13, 2013
Last update date Apr 26, 2013
Contact name Grzegorz Kudla
E-mail(s) gkudla@gmail.com
Phone +44 (0) 131 332 2471
Organization name University of Edinburgh
Department MRC HGU
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
 
Platform ID GPL5188
Series (1)
GSE46039 hsa-miR-92a knock down in Flp-in T-REx 293-PTH-AGO1 cells

Data table header descriptions
ID_REF
VALUE RMA signal estimates

Data table
ID_REF VALUE
2315252 7.391737
2315253 3.579214
2315374 8.462379
2315375 5.975938
2315376 8.327979
2315377 7.561098
2315586 7.996964
2315587 10.40683
2315588 8.764039
2315589 9.216775
2315591 8.44867
2315594 8.714549
2315595 5.273171
2315596 7.498546
2315598 7.374793
2315602 7.818693
2315603 9.474319
2315604 8.678077
2315605 10.9003
2315606 9.05689

Total number of rows: 287329

Table truncated, full table size 4739 Kbytes.




Supplementary file Size Download File type/resource
GSM1122226_Tollervey_R6_CTRL_HuEx-1_0-st-v2_.CEL.gz 24.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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