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Sample GSM1122221 Query DataSets for GSM1122221
Status Public on Apr 26, 2013
Title 293_AMO-miR-92_rep5
Sample type RNA
 
Source name Flp-in T-REx 293-PTH-AGO1 AMO-miR-92a treated
Organism Homo sapiens
Characteristics treatment: miR-92a inhibitor
cell line: Flp-in T-REx 293-PTH-AGO1
Treatment protocol Flp-In T-REx 293-PTH-AGO1 cells were transfected with Lipofectamine 2000 (Life Technologies) and miR-92a (mA/ZEN/mCmAmGmGmCmCmGmGmGmAmCmAmAmGmUmGmCmA mAmU/ZEN/mA; IDT; 6.25nM) or control inhibitor (mC/ZEN/mGmCmGmAmCmUmAmUmAmCmGmCmGmCmAmAmUmAmUmGmG/ZEN/mU; IDT; 6.25nM)
Growth protocol Flp-In T-REx 293 cells were stably transfected with a plasmid encoding AGO1 N-terminally tagged with ProteinA-TEV protease cleavage site-Hisx6. Expression of tagged AGO1 is tetracycline inducible. For the purpose of this microarray analysis AGO1 expression was not induced. Cells were grown in Dulbecco’s modified Eagle medium (DMEM) with high glucose, supplemented with 10% fetal bovine serum at 37°C under humidified air with 5% CO2
Extracted molecule total RNA
Extraction protocol total RNA was izolated 48 hours post transfecton with TRIZOL (Life Technologies) according to manufacturers instructions; sample integrity was confirmed on Bioanalyzer and samples were further processed by the Source BioScience UK Limited
Label biotin
Label protocol sense strand cDNA was prepared with Ambion WT Expression Kit (Life Technologies, 4411973), fragmentation and biotin labelling was done with GeneChip WT terminal labelling kit (Affymetrix, 900670)
 
Hybridization protocol fragmented and labelled cDNA was hybridized to GeneChip® Human Exon 1.0 ST (Affymetrix,900694) at 45°C for 16 hours, washing and staining was done using GeneChipHybridization Wash and Stain kit (Affymetrix, 900720)
Scan protocol hybridized microarrays were scanned on GeneChip Scanner 3000 (Affymetrix, P7N 00-00212)
Data processing RMA-EXON-CORE-DABG protocol
HuEx-1_0-st-v2.r2.pgf
HuEx-1_0-st-v2.r2.dt1.hg18.core.mps
 
Submission date Apr 13, 2013
Last update date Apr 26, 2013
Contact name Grzegorz Kudla
E-mail(s) gkudla@gmail.com
Phone +44 (0) 131 332 2471
Organization name University of Edinburgh
Department MRC HGU
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
 
Platform ID GPL5188
Series (1)
GSE46039 hsa-miR-92a knock down in Flp-in T-REx 293-PTH-AGO1 cells

Data table header descriptions
ID_REF
VALUE RMA signal estimates

Data table
ID_REF VALUE
2315252 7.720447
2315253 4.362236
2315374 8.405762
2315375 6.807776
2315376 8.549595
2315377 7.86857
2315586 8.218382
2315587 10.2358
2315588 8.744323
2315589 9.094418
2315591 8.581989
2315594 8.721011
2315595 5.80653
2315596 7.47373
2315598 7.638055
2315602 7.529391
2315603 9.478059
2315604 8.494389
2315605 10.93773
2315606 9.03135

Total number of rows: 287329

Table truncated, full table size 4739 Kbytes.




Supplementary file Size Download File type/resource
GSM1122221_Tollervey_R3_92_HuEx-1_0-st-v2_.CEL.gz 24.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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