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Sample GSM1122219 Query DataSets for GSM1122219
Status Public on Apr 26, 2013
Title 293_AMO-miR-92_rep3
Sample type RNA
Source name Flp-in T-REx 293-PTH-AGO1 AMO-miR-92a treated
Organism Homo sapiens
Characteristics treatment: miR-92a inhibitor
cell line: Flp-in T-REx 293-PTH-AGO1
Treatment protocol Flp-In T-REx 293-PTH-AGO1 cells were transfected with Lipofectamine 2000 (Life Technologies) and miR-92a (mA/ZEN/mCmAmGmGmCmCmGmGmGmAmCmAmAmGmUmGmCmA mAmU/ZEN/mA; IDT; 6.25nM) or control inhibitor (mC/ZEN/mGmCmGmAmCmUmAmUmAmCmGmCmGmCmAmAmUmAmUmGmG/ZEN/mU; IDT; 6.25nM)
Growth protocol Flp-In T-REx 293 cells were stably transfected with a plasmid encoding AGO1 N-terminally tagged with ProteinA-TEV protease cleavage site-Hisx6. Expression of tagged AGO1 is tetracycline inducible. For the purpose of this microarray analysis AGO1 expression was not induced. Cells were grown in Dulbecco’s modified Eagle medium (DMEM) with high glucose, supplemented with 10% fetal bovine serum at 37°C under humidified air with 5% CO2
Extracted molecule total RNA
Extraction protocol total RNA was izolated 48 hours post transfecton with TRIZOL (Life Technologies) according to manufacturers instructions; sample integrity was confirmed on Bioanalyzer and samples were further processed by the Source BioScience UK Limited
Label biotin
Label protocol sense strand cDNA was prepared with Ambion WT Expression Kit (Life Technologies, 4411973), fragmentation and biotin labelling was done with GeneChip WT terminal labelling kit (Affymetrix, 900670)
Hybridization protocol fragmented and labelled cDNA was hybridized to GeneChip® Human Exon 1.0 ST (Affymetrix,900694) at 45°C for 16 hours, washing and staining was done using GeneChipHybridization Wash and Stain kit (Affymetrix, 900720)
Scan protocol hybridized microarrays were scanned on GeneChip Scanner 3000 (Affymetrix, P7N 00-00212)
Data processing RMA-EXON-CORE-DABG protocol
Submission date Apr 13, 2013
Last update date Apr 26, 2013
Contact name Grzegorz Kudla
Phone +44 (0) 131 332 2471
Organization name University of Edinburgh
Department MRC HGU
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
Platform ID GPL5188
Series (1)
GSE46039 hsa-miR-92a knock down in Flp-in T-REx 293-PTH-AGO1 cells

Data table header descriptions
VALUE RMA signal estimates

Data table
2315252 7.559577
2315253 3.923049
2315374 8.842853
2315375 6.823245
2315376 8.111716
2315377 8.161224
2315586 8.474908
2315587 10.39005
2315588 9.120595
2315589 9.09995
2315591 8.405334
2315594 8.706079
2315595 5.502161
2315596 7.847784
2315598 7.732861
2315602 7.708841
2315603 9.44185
2315604 8.760291
2315605 11.04338
2315606 9.114805

Total number of rows: 287329

Table truncated, full table size 4739 Kbytes.

Supplementary file Size Download File type/resource
GSM1122219_Tollervey_R1_92_HuEx-1_0-st-v2_.CEL.gz 25.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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