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Sample GSM1122218 Query DataSets for GSM1122218
Status Public on Apr 26, 2013
Title 293_AMO-miR-92_rep2
Sample type RNA
Source name Flp-in T-REx 293-PTH-AGO1 AMO-miR-92a treated
Organism Homo sapiens
Characteristics treatment: miR-92a inhibitor
cell line: Flp-in T-REx 293-PTH-AGO1
Treatment protocol Flp-In T-REx 293-PTH-AGO1 cells were transfected with Lipofectamine 2000 (Life Technologies) and miR-92a (mA/ZEN/mCmAmGmGmCmCmGmGmGmAmCmAmAmGmUmGmCmA mAmU/ZEN/mA; IDT; 6.25nM) or control inhibitor (mC/ZEN/mGmCmGmAmCmUmAmUmAmCmGmCmGmCmAmAmUmAmUmGmG/ZEN/mU; IDT; 6.25nM)
Growth protocol Flp-In T-REx 293 cells were stably transfected with a plasmid encoding AGO1 N-terminally tagged with ProteinA-TEV protease cleavage site-Hisx6. Expression of tagged AGO1 is tetracycline inducible. For the purpose of this microarray analysis AGO1 expression was not induced. Cells were grown in Dulbecco’s modified Eagle medium (DMEM) with high glucose, supplemented with 10% fetal bovine serum at 37°C under humidified air with 5% CO2
Extracted molecule total RNA
Extraction protocol total RNA was izolated 48 hours post transfecton with TRIZOL (Life Technologies) according to manufacturers instructions; sample integrity was confirmed on Bioanalyzer and samples were further processed by the Source BioScience UK Limited
Label biotin
Label protocol sense strand cDNA was prepared with Ambion WT Expression Kit (Life Technologies, 4411973), fragmentation and biotin labelling was done with GeneChip WT terminal labelling kit (Affymetrix, 900670)
Hybridization protocol fragmented and labelled cDNA was hybridized to GeneChip® Human Exon 1.0 ST (Affymetrix,900694) at 45°C for 16 hours, washing and staining was done using GeneChipHybridization Wash and Stain kit (Affymetrix, 900720)
Scan protocol hybridized microarrays were scanned on GeneChip Scanner 3000 (Affymetrix, P7N 00-00212)
Data processing RMA-EXON-CORE-DABG protocol
Submission date Apr 13, 2013
Last update date Apr 26, 2013
Contact name Grzegorz Kudla
Phone +44 (0) 131 332 2471
Organization name University of Edinburgh
Department MRC HGU
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
Platform ID GPL5188
Series (1)
GSE46039 hsa-miR-92a knock down in Flp-in T-REx 293-PTH-AGO1 cells

Data table header descriptions
VALUE RMA signal estimates

Data table
2315252 7.839778
2315253 4.6739
2315374 8.474679
2315375 6.780951
2315376 8.200302
2315377 7.334842
2315586 8.07742
2315587 10.23321
2315588 8.942593
2315589 9.184373
2315591 8.404604
2315594 8.522026
2315595 4.092476
2315596 7.615936
2315598 7.303165
2315602 7.451893
2315603 9.345073
2315604 8.573966
2315605 10.78741
2315606 8.848286

Total number of rows: 287329

Table truncated, full table size 4738 Kbytes.

Supplementary file Size Download File type/resource
GSM1122218_Tollervey_G2_92_HuEx-1_0-st-v2_.CEL.gz 24.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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