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Sample GSM1122217 Query DataSets for GSM1122217
Status Public on Apr 26, 2013
Title 293_AMO-miR-92_rep1
Sample type RNA
 
Source name Flp-in T-REx 293-PTH-AGO1 AMO-miR-92a treated
Organism Homo sapiens
Characteristics treatment: miR-92a inhibitor
cell line: Flp-in T-REx 293-PTH-AGO1
Treatment protocol Flp-In T-REx 293-PTH-AGO1 cells were transfected with Lipofectamine 2000 (Life Technologies) and miR-92a (mA/ZEN/mCmAmGmGmCmCmGmGmGmAmCmAmAmGmUmGmCmA mAmU/ZEN/mA; IDT; 6.25nM) or control inhibitor (mC/ZEN/mGmCmGmAmCmUmAmUmAmCmGmCmGmCmAmAmUmAmUmGmG/ZEN/mU; IDT; 6.25nM)
Growth protocol Flp-In T-REx 293 cells were stably transfected with a plasmid encoding AGO1 N-terminally tagged with ProteinA-TEV protease cleavage site-Hisx6. Expression of tagged AGO1 is tetracycline inducible. For the purpose of this microarray analysis AGO1 expression was not induced. Cells were grown in Dulbecco’s modified Eagle medium (DMEM) with high glucose, supplemented with 10% fetal bovine serum at 37°C under humidified air with 5% CO2
Extracted molecule total RNA
Extraction protocol total RNA was izolated 48 hours post transfecton with TRIZOL (Life Technologies) according to manufacturers instructions; sample integrity was confirmed on Bioanalyzer and samples were further processed by the Source BioScience UK Limited
Label biotin
Label protocol sense strand cDNA was prepared with Ambion WT Expression Kit (Life Technologies, 4411973), fragmentation and biotin labelling was done with GeneChip WT terminal labelling kit (Affymetrix, 900670)
 
Hybridization protocol fragmented and labelled cDNA was hybridized to GeneChip® Human Exon 1.0 ST (Affymetrix,900694) at 45°C for 16 hours, washing and staining was done using GeneChipHybridization Wash and Stain kit (Affymetrix, 900720)
Scan protocol hybridized microarrays were scanned on GeneChip Scanner 3000 (Affymetrix, P7N 00-00212)
Data processing RMA-EXON-CORE-DABG protocol
HuEx-1_0-st-v2.r2.pgf
HuEx-1_0-st-v2.r2.dt1.hg18.core.mps
 
Submission date Apr 13, 2013
Last update date Apr 26, 2013
Contact name Grzegorz Kudla
E-mail(s) gkudla@gmail.com
Phone +44 (0) 131 332 2471
Organization name University of Edinburgh
Department MRC HGU
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
 
Platform ID GPL5188
Series (1)
GSE46039 hsa-miR-92a knock down in Flp-in T-REx 293-PTH-AGO1 cells

Data table header descriptions
ID_REF
VALUE RMA signal estimates

Data table
ID_REF VALUE
2315252 7.860203
2315253 4.296054
2315374 8.447074
2315375 5.749037
2315376 8.282156
2315377 7.884662
2315586 8.025409
2315587 10.11291
2315588 8.957661
2315589 9.150916
2315591 8.371619
2315594 8.472173
2315595 5.310365
2315596 7.385644
2315598 7.614397
2315602 7.559979
2315603 9.392161
2315604 8.469501
2315605 10.68832
2315606 8.850315

Total number of rows: 287329

Table truncated, full table size 4738 Kbytes.




Supplementary file Size Download File type/resource
GSM1122217_Tollervey_G1_92_HuEx-1_0-st-v2_.CEL.gz 24.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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