|
| Status |
Public on Apr 21, 2014 |
| Title |
Bacteroides thetaiotaomicron response in cecum of Fut2_KO mouse fed with polysaccharide-rich diet rep3 |
| Sample type |
RNA |
| |
|
| Source name |
BT_Fut2_KO mouse cecum_polysaccharide-rich diet
|
| Organism |
Bacteroides thetaiotaomicron |
| Characteristics |
strain: VPI-5482
host/mouse strain: C57BL/6J
host/mouse genotype: Fut2_KO
host fed with: polysaccharide-rich diet
bacteria harvested from: BT-colonized mice cecum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy mini column extraction, Ambion DNA free treatment
|
| Label |
Biotin
|
| Label protocol |
Enzo Biochemicals Bioarray terminal labeling kit
|
| |
|
| Hybridization protocol |
Affymetrix
|
| Scan protocol |
Affymetrix
|
| Description |
BT_Fut2_KO_SD_3
|
| Data processing |
The raw data were RMA-MS normalized using R-2.9.1. and log2 transformed, excluding other species present on chip.
|
| |
|
| Submission date |
Mar 29, 2013 |
| Last update date |
Apr 21, 2014 |
| Contact name |
Angela M Marcobal |
| E-mail(s) |
marcobal@stanford.edu
|
| Phone |
1-650-721-2961
|
| Fax |
1-650-498-7147
|
| URL |
http://sonnenburglab.stanford.edu/index.html
|
| Organization name |
Stanford University
|
| Department |
Microbiology and Immunology
|
| Lab |
Justin Sonnenburg lab
|
| Street address |
299 Campus Drive
|
| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL1821 |
| Series (1) |
| GSE45641 |
Intestine-adapted bacterial symbiont response to polysaccharide rich and polysaccharide deficient diets in mice lacking fucosyltransferase-2 |
|
