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Sample GSM1099033 Query DataSets for GSM1099033
Status Public on Mar 16, 2013
Title TCF7L2 ChIP-seq vehicle treatment
Sample type SRA
Source name TCF7L2 ChIP-seq vehicle treatment
Organism Homo sapiens
Characteristics cell line: MDA-MB-453
cell type: breast cancer
chip antibody: sc-8631 Santa Cruz
Treatment protocol The MDA-MB-453 cells were grown in the phenol red-free medium supplemented with 5% charcoal/dextran-treated fetal bovine serum for 3 days, followed by treatment of vehicle or 10 nM DHT for the indicated time.
Growth protocol The MDA-MB-453 cells were maintained in DMEM supplemented with 10% fetal bovine serum at 37oC incubator
Extracted molecule genomic DNA
Extraction protocol Lysates were clarified from sonicated cells and the transcription factor-bound complexes were isolated with antibody.
Libraries were prepared according to Illumina's instructions with 10 ng of ChIP-DNA.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
Description ChIP-seq data
Data processing Basecalls performed using CASAVA version 1.9
ChIP-seq reads were aligned to the hg18 genome assembly using Bowtie-0.12.7
peaks were called using MACS2
Genome_build: hg18
Supplementary_files_format_and_content: peak files represent the boundary of each ChIP-enriched region are in the BED format
Submission date Mar 15, 2013
Last update date May 15, 2019
Contact name Yiwen Chen
Organization name University of Texas MD Anderson Cancer Center
Department Bioinformatics and Computational Biology
Lab Yiwen Chen
Street address 1400 Pressler Street
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platform ID GPL11154
Series (2)
GSE45201 Amplitude modulation of androgen signaling by c-MYC [ChIP-Seq]
GSE45203 Amplitude modulation of androgen signaling by c-MYC
SRA SRX250095
BioSample SAMN01978924

Supplementary file Size Download File type/resource
GSM1099033_TCF7L2-vehicle-peak.bed.gz 96.2 Kb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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