|
| Status |
Public on Apr 01, 2013 |
| Title |
SNDISH15, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
SND1 knockdown 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: QGY-7703
cell type: human hepatocellular carcinoma
shRNA: SND1
|
| Treatment protocol |
No treatment was done.
|
| Growth protocol |
Steady-state proliferating cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed using QIAGEN columns according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (GeneChip 3' IVT Express Kit, P/N 702646 Rev. 8, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using protocol FS450_0002.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
SNDISH15_2
Gene expression data from SND1 knockdown 2.
|
| Data processing |
The data were analyzed with Affymetrix Expression Console software using Affymetrix default analysis settings and RMA as the normalization method.
|
| |
|
| Submission date |
Feb 23, 2013 |
| Last update date |
Apr 01, 2013 |
| Contact name |
Myrna Serrano |
| Organization name |
Virginia Commonwealth University
|
| Lab |
NARF
|
| Street address |
1101 East Marshall St
|
| City |
Richmond |
| State/province |
VA |
| ZIP/Postal code |
23298 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE44601 |
Expression data from SND1 knockdown clones of human HCC cell line QGY-7703 |
|
