|
| Status |
Public on Sep 02, 2015 |
| Title |
E11.5 heart, Input |
| Sample type |
SRA |
| |
|
| Source name |
E11.5 heart, Input
|
| Organism |
Mus musculus |
| Characteristics |
strain: Parkes
developmental stage: E11.5
tissue: heart
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Fixed embryonic hearts were homogenised using an Ultra-Turrax, sonicated and Nkx2-5-DNA complexes were isolated using NKX-2.5 (N-19) sc-8697 antibody from Santa Cruz.
Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
Input
|
| Data processing |
Basecalls performed using CASAVA version 1.4
ChIP-seq reads were aligned to the mm9 genome assembly using Bowtie.
peaks were called using MACS1.4, p-value=1x10-14
Genome_build: mm9
Supplementary_files_format_and_content: .bed files containing MACS peaks
|
| |
|
| Submission date |
Feb 22, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Laurent Dupays |
| E-mail(s) |
laurent.dupays@crick.ac.uk
|
| Organization name |
The Francis Crick Institute
|
| Lab |
Mill Hill Laboratory
|
| Street address |
The Ridgeway, Mill Hill
|
| City |
London |
| ZIP/Postal code |
NW7 1AA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL11002 |
| Series (2) |
| GSE44574 |
Genome-wide maps of Nkx2-5 in E11.5 mouse embryonic heart |
| GSE44576 |
Identification of NKX2-5 transcriptional targets in the mouse embryonic heart |
|
| Relations |
| SRA |
SRX244285 |
| BioSample |
SAMN01924407 |
