|
Status |
Public on Feb 12, 2013 |
Title |
4M_AlcoholvsControl |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
pooled RNA from six alcohol-treated mice
|
Organism |
Mus musculus |
Characteristics |
tissue: liver time-point: 4 month treatment strain: 129/Sv Sex: male treatment: alcohol-treated
|
Treatment protocol |
Alcohol-treated mice were fed ad libitum a liquid diet containing 4% ethanol. Control animals were fed an isocaloric diet supplemented with maltose dextran
|
Growth protocol |
Mice were 6-8 weeks old when the treatment started
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol followed by RNeasy Midi kit
|
Label |
Cy3
|
Label protocol |
Invitrogen SuperScript indirect cDNA labeling system
|
|
|
Channel 2 |
Source name |
pooled RNA from six control mice
|
Organism |
Mus musculus |
Characteristics |
tissue: liver time-point: 4 month treatment strain: 129/Sv Sex: male treatment: none
|
Treatment protocol |
Alcohol-treated mice were fed ad libitum a liquid diet containing 4% ethanol. Control animals were fed an isocaloric diet supplemented with maltose dextran
|
Growth protocol |
Mice were 6-8 weeks old when the treatment started
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol followed by RNeasy Midi kit
|
Label |
Cy5
|
Label protocol |
Invitrogen SuperScript indirect cDNA labeling system
|
|
|
|
Hybridization protocol |
follow Agilent's two-color expression array hybridization protocol
|
Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version 9.1).
|
Data processing |
Feature Extraction version 9.1was used to filter, normalize, and calculate the signal intensities and Cy5/Cy3 ratios.
|
|
|
Submission date |
Feb 11, 2013 |
Last update date |
Feb 12, 2013 |
Contact name |
Henghong Li |
E-mail(s) |
hl234@georgetown.edu
|
Organization name |
Georgetown University
|
Department |
Biochemistry
|
Lab |
Fornace
|
Street address |
3970 Reservoir Rd., NW
|
City |
Washington |
State/province |
DC |
ZIP/Postal code |
20057 |
Country |
USA |
|
|
Platform ID |
GPL7202 |
Series (1) |
GSE44237 |
Gene expression changes in liver tissues from alcohol treated mice |
|