|
| Status |
Public on Feb 12, 2013 |
| Title |
4M_AlcoholvsControl |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
pooled RNA from six alcohol-treated mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
time-point: 4 month treatment
strain: 129/Sv
Sex: male
treatment: alcohol-treated
|
| Treatment protocol |
Alcohol-treated mice were fed ad libitum a liquid diet containing 4% ethanol. Control animals were fed an isocaloric diet supplemented with maltose dextran
|
| Growth protocol |
Mice were 6-8 weeks old when the treatment started
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol followed by RNeasy Midi kit
|
| Label |
Cy3
|
| Label protocol |
Invitrogen SuperScript indirect cDNA labeling system
|
| |
|
| Channel 2 |
| Source name |
pooled RNA from six control mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
time-point: 4 month treatment
strain: 129/Sv
Sex: male
treatment: none
|
| Treatment protocol |
Alcohol-treated mice were fed ad libitum a liquid diet containing 4% ethanol. Control animals were fed an isocaloric diet supplemented with maltose dextran
|
| Growth protocol |
Mice were 6-8 weeks old when the treatment started
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol followed by RNeasy Midi kit
|
| Label |
Cy5
|
| Label protocol |
Invitrogen SuperScript indirect cDNA labeling system
|
| |
|
| |
| Hybridization protocol |
follow Agilent's two-color expression array hybridization protocol
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version 9.1).
|
| Data processing |
Feature Extraction version 9.1was used to filter, normalize, and calculate the signal intensities and Cy5/Cy3 ratios.
|
| |
|
| Submission date |
Feb 11, 2013 |
| Last update date |
Feb 12, 2013 |
| Contact name |
Henghong Li |
| E-mail(s) |
hl234@georgetown.edu
|
| Organization name |
Georgetown University
|
| Department |
Biochemistry
|
| Lab |
Fornace
|
| Street address |
3970 Reservoir Rd., NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20057 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE44237 |
Gene expression changes in liver tissues from alcohol treated mice |
|
