|
| Status |
Public on Apr 10, 2013 |
| Title |
Sarcoma cell line - Dicer Null -rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Sarcoma cell line Null for Dicer
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Sarcoma cell line
strain: C57BL6/J
genotype/variation: Dicer Null
|
| Treatment protocol |
Dicer-heterozygous and Dicer KO cells were grown to confluence in T25s and harvested for extraction of total RNA.
|
| Growth protocol |
Cells were cultured in DMEM supplemented with penicillin/streptomycin, L-glutamine, and 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430_2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Data processing |
R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.0, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.0, Biobase_2.8.0
|
| |
|
| Submission date |
Feb 07, 2013 |
| Last update date |
Apr 10, 2013 |
| Contact name |
A Bhutkar |
| Organization name |
MIT
|
| Street address |
77 Massachusetts Avenue
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE44162 |
Let-7 represses Nr6a1 and a mid-gestation developmental program in adult fibroblasts [3p arrays] |
| GSE44163 |
Let-7 represses Nr6a1 and a mid-gestation developmental program in adult fibroblasts |
|
