|
| Status |
Public on Jan 09, 2013 |
| Title |
Lymphnode_6 hr_GLA-SE_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Lymphnode
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/c
tissue: Lymphnode
agent: GLA-SE
time: 6 hr
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolation using RNeasy Mini Kit (Qiagen cat #: 74104) or RNeasy Protect Mini Kit (Qiagen cat #: 74124) followed by GLOBINclear™ Protocol (Invitrogen cat #: AM1981) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 400 ng total RNA (Expression Analysis Technical Manual, Rev 3, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on mouse genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
ACF Study Number: 078-11-008
SAMPLE 11
|
| Data processing |
The data were analyzed with Expression Console Software using Affymetrix default analysis settings (RMA) and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 09, 2013 |
| Last update date |
Jan 09, 2013 |
| Contact name |
Zheng Liu |
| Organization name |
Medimmune LLC
|
| Street address |
One Medimmune Way
|
| City |
Gaithersburg |
| ZIP/Postal code |
20878 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE43373 |
Molecular and cellular response profiles induced by the TLR4 agonist-based adjuvant Glucopyranosyl Lipid A |
|
