Brian Welm and Brittni Smith - Cancer Institute University of Utah
Treatment protocol
described in Hu et al., 2005, BioTechniques, 38:121-124
Growth protocol
described in Hu et al., 2005, BioTechniques, 38:121-124
Extracted molecule
total RNA
Extraction protocol
Qiagen Rneasy Kit
Label
Cy5
Label protocol
described in Hu et al., 2005, BioTechniques, 38:121-124
Hybridization protocol
described in Hu et al., 2005, BioTechniques, 38:121-124
Scan protocol
Agilent Technologies Scanner G2505C US82800149 Using Feature Extraction Software
Data processing
Array data was filtered to remove control features and any non-uniform features or population outlier features. Remaining features were averaged to yield a single value for each unique probe. Finally data were log2 transformed, quantile normalized, and adjusted for batch effects using ComBat.
