|
| Status |
Public on Dec 01, 2013 |
| Title |
Anti-GFP_control KD_1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse ES cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129S4/Svjae
phenotype: agouti
gender: male
cell type: ESC
genotype/variation: control
|
| Treatment protocol |
mESCs were collected through trypsinization, washed with PBS twice, followed by RNA extraction
|
| Growth protocol |
mESCs were grown in standard mouse ES cells media with LIF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNAeasy plus mini kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Anti-GFP shRNA KD in mESCs
|
| Data processing |
The data were analyzed by dchip and arrays were normalized against the baseline array that has the median brightness.
|
| |
|
| Submission date |
Dec 20, 2012 |
| Last update date |
Dec 01, 2013 |
| Contact name |
Zhen Shao |
| E-mail(s) |
shaozhen@picb.ac.cn
|
| Organization name |
Partner Institute for Computational Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences
|
| Lab |
Zhen Shao
|
| Street address |
320 Yueyang Road
|
| City |
Shanghai |
| ZIP/Postal code |
230031 |
| Country |
China |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE43059 |
Role of Jmjd2b and Jmjd2c in mESCs identity |
| GSE43231 |
Combinatorial role of Jmjd2b and Jmjd2c in mESC identity |
|
