|
| Status |
Public on Feb 09, 2013 |
| Title |
Ldb1+/+, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
hemangioblast equivalent cells
|
| Organism |
Mus musculus |
| Characteristics |
cell-type: Ldb1+/+ from Flk1+ cells
genotype: Ldb1+/+
age: Flk1+ BL-CFCs isolated from day 4 embryoid bodies
|
| Growth protocol |
Ldb1+/+ and Ldb1-/- ES cells were grown in suspension at 10.000 cells/ml on nonadherent dishes in IMDM medium (without Leukemia Inhibitory Factor (LIF)) with 15% FCS, 1% P/S, 1% L-glutamine (Gibco, Cat.25030-08), 0.05μg/ml transferrin (Roche, Cat.652-202), 0.05μg/ml ascorbic acid (Sigma, Cat.A-4544), 3μl/ml monothioglycerol (Sigma, Cat.M-6145) for day 4 EBs and 1.8μl/ml for day 6 and day 8 EBs. 5% protein free hybridoma medium II (Gibco, Cat.12040-077) was added for day 6 and day 8 EBs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from Ldb1+/+ and Ldb1-/- Flk1+ cells with the QIAGEN RNeasy Mini Kit and integrity was checked on the Agilent 2100 Bioanalyzer.
|
| Label |
biotin-labelled cRNA
|
| Label protocol |
RNA was converted to biotin-labelled cRNA, hybridised on the Mouse Genome 430 2.0 Array
|
| |
|
| Hybridization protocol |
Hybridization was performed by the affymetrix standard protocol on the Mouse Genome 430 2.0 Array
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000 according to the manufacturer protocol.
|
| Description |
Gene expression data from hemangioblast in vitro equivalent cells with Ldb1 +/+.
|
| Data processing |
Affymetrix GeneChip® Expression Arrays were normalized with the standard RMA protocol by the Affy Bioconductor package
|
| |
|
| Submission date |
Dec 19, 2012 |
| Last update date |
Feb 09, 2013 |
| Contact name |
Supat Thongjuea |
| E-mail(s) |
supat.thongjuea@ndcls.ox.ac.uk
|
| Organization name |
The Weatherall Institute of Molecular Medicine
|
| Department |
MRC Molecular Haematology Unit
|
| Street address |
Headington
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE43042 |
The role of Ldb1 in hemangioblast development: genome-wide analysis shows that Ldb1 controls essential hematopoietic genes/pathways in mouse early development and reveals novel players in hematopoiesis (Affymetrix) |
| GSE43044 |
The role of Ldb1 in hemangioblast development: genome-wide analysis shows that Ldb1 controls essential hematopoietic genes/pathways in mouse early development and reveals novel players in hematopoiesis |
|
