|
| Status |
Public on Jan 15, 2013 |
| Title |
Exponential |
| Sample type |
SRA |
| |
|
| Source name |
Mouse Embryonic Fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Fibroblasts
developmental stage: E13.5
strain: C57BL/6
antibody: S17S anti-Erf rabbit polyclonal antibody
|
| Treatment protocol |
For serum starvation, 90%-confluent cultures were grown for 4 hours in DMEM supplemented with 20mM HEPES ph7
|
| Growth protocol |
Mouse embryo fibroblasts were derived from E13.5 C57/BL6 embryos after removal of the head, heart and liver and outgrowth for 15 days in DMEM supplemented with10%FCS.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were fixed with 1% formaldehyde in PBS for 10 min at RT. Erf-DNA complexes were immunoprecipitated from sonicated nuclear extracts with the S17S anti-Erf rabbit polyclonal antibody
Standard Illumina Paired End
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Paired-end reads from ChIP-Seq and input samples were aligned to the mouse genome build using Bowtie (version 0.12.3) using standard parameters
Wig files of genomic read distribution were produced using a sliding 300bp window moving at 30 bp increments
Genome_build: mm9
Supplementary_files_format_and_content: Wig files of genomic distribution of aligned reads
|
| |
|
| Submission date |
Dec 14, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Stephen Taylor |
| E-mail(s) |
stephen.taylor@imm.ox.ac.uk
|
| Phone |
+44 1865 222640
|
| Organization name |
CBRG
|
| Street address |
Headington
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE42936 |
Erf chromatin binding-sites in E13.5 mouse embryo fibroblasts |
|
| Relations |
| SRA |
SRX209938 |
| BioSample |
SAMN01831308 |
